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Literature DB >> 6310509

The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.

Abstract

The nucleotide sequence of 1100bp around the origin of replication of the pSC101 plasmid has been determined. This segment of DNA is capable of replication in the presence of a helper plasmid. The sequence data reveal similarities between pSC101 and several other replicons. The origin of replication contains three direct repeats of an 18bp sequence associated with a segment exceptionally rich in A-T base pairs. A promotor that probably directs transcription of a gene encoding an essential plasmid replication function is associated with a region of extensive potential secondary structure. The sequence presented here includes the sequence of the par region involved in partitioning of plasmids at cell division.

Entities: Gene Species

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Year: 1983 PMID： 6310509 PMCID： PMC326303 DOI： 10.1093/nar/11.16.5645

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

47 in total

The nucleotide sequence of replication and maintenance functions encoded by plasmid pSC101.

1. Base pairing of RNA I with its complementary sequence in the primer precursor inhibits ColE1 replication.

2. RNAs involved in copy-number control and incompatibility of plasmid R1.

3. Enzymatic replication of the origin of the Escherichia coli chromosome.

4. Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid.

5. Nucleotide sequence of the O gene and of the origin of replication in bacteriophage lambda DNA.

6. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

7. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

8. Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript.

9. Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands.

10. Direct repeats of the F plasmid incC region express F incompatibility.

1. Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator protein.

2. New pSC101-derivative cloning vectors with elevated copy numbers.

Review 3. Replication and control of circular bacterial plasmids.

4. Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.

5. Requirement of the Escherichia coli dnaA gene function for ori-2-dependent mini-F plasmid replication.

6. Transcription events in the origin of replication of plasmid pSC101.

7. Nucleotide sequence and functional properties of DNA encoding incompatibility in the broad host-range plasmid R1162.

8. In vivo and in vitro studies of a copy number mutation of the RepA replication protein of plasmid pSC101.

9. Host participation in plasmid maintenance: dependence upon dnaA of replicons derived from P1 and F.

10. Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF.