Literature DB >> 6309948

Ultrastructural localization of 5'-nucleotidase in guinea pig neutrophils based upon the use of cerium as capturing agent.

J M Robinson, M J Karnovsky.   

Abstract

A cytochemical method for 5'-nucleotidase localization in which cerium serves as the capture agent in order to enzymatically detect liberated inorganic phosphate has been developed. The method has been established in cell-free model systems and in guinea pig neutrophils where 5'-nucleotidase is restricted to the plasmalemma as an ectoenzyme. This cerium-based method gives better results for ultrastructural localization of 5'-nucleotidase than conventional lead-based methods.

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Year:  1983        PMID： 6309948     DOI： 10.1177/31.10.6309948

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  19 in total

Review 1.  5'-Nucleotidase: molecular structure and functional aspects.

Authors:  H Zimmermann
Journal:  Biochem J       Date:  1992-07-15       Impact factor: 3.857

2.  A simple method for ultrastructural enzyme cytochemistry on small volumes and numbers of isolated cells.

Authors:  J M Robinson
Journal:  Histochem J       Date:  1991-01

Review 3.  Reactive oxygen species in phagocytic leukocytes.

Authors:  John M Robinson
Journal:  Histochem Cell Biol       Date:  2008-07-03       Impact factor: 4.304

4.  Parathyroid cell polarity as revealed by cytochemical localization of ATPases, alkaline phosphatase and 5'-nucleotidase.

Authors:  P Wild; E M Schraner
Journal:  Histochemistry       Date:  1990

5.  Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure.

Authors:  J Song; K S Bosch; W Tigchelaar; R J Van Den Munckhof; J P Schellens; C J Van Noorden; W M Frederiks
Journal:  Histochem J       Date:  1995-11

6.  Demonstration of the intralobular lymphatics in the guinea pig pancreas by an enzyme-histochemical method.

Authors:  R C Ji; S Kato
Journal:  J Anat       Date:  1997-07       Impact factor: 2.610

7.  Localization of four phosphatases in rat liver sinusoidal cells. An enzyme cytochemical study.

Authors:  V De Valck; A Geerts; P Schellinck; E Wisse
Journal:  Histochemistry       Date:  1988

8.  Electron microscopic localization of 5'-nucleotidase in rat salivary glands. Comparative enzyme- and immunohistochemical studies.

Authors:  S Yamashina; O Katsumata; I Wada; K Kato
Journal:  Histochemistry       Date:  1986

9.  Autoradiography-based cytochemical detection of ecto-ATPase, ecto-ADPase, 5'-nucleotidase, and extracellular adenosine production, employing 141Ce3+ as a capturing agent.

Authors:  O Culic; R Lemmens; H Teuchy; L Vanduffel
Journal:  Histochem J       Date:  1995-07

10.  Reconstitution of 5'-nucleotidase of bull seminal plasma in spin-labeled liposomes.

Authors:  C Fini; V D Thuong; M Aliante; A Floridi; S Cannistraro
Journal:  J Membr Biol       Date:  1994-10       Impact factor: 1.843
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