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Literature DB >> 630809

A radioimmunoassay for 1,25-dihydroxycholecalciferol.

T L Clemens, G N Hendy, R F Graham, E G Baggiolini, M R Uskokovic, J L O'Riordan.

Abstract

1. 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.

Entities: Chemical Species

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Year: 1978 PMID： 630809 DOI： 10.1042/cs0540329

Source DB: PubMed Journal: Clin Sci Mol Med ISSN： 0301-0538

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Cited

9 in total

1. The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going.

Authors: Bruce W Hollis; Ronald L Horst
Journal: J Steroid Biochem Mol Biol Date: 2007-01-02 Impact factor: 4.292

Review 2. The clinical use of vitamin D metabolites and their potential developments: a position statement from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) and the International Osteoporosis Foundation (IOF).

Authors: Luisella Cianferotti; Claudio Cricelli; John A Kanis; Ranuccio Nuti; Jean-Y Reginster; Johann D Ringe; Rene Rizzoli; Maria Luisa Brandi
Journal: Endocrine Date: 2015-05-01 Impact factor: 3.633

3. Regulation of human tonsillar T-cell proliferation by the active metabolite of vitamin D3.

Authors: J D Nunn; D R Katz; S Barker; L J Fraher; M Hewison; G N Hendy; J L O'Riordan
Journal: Immunology Date: 1986-12 Impact factor: 7.397

Review 4. Assessment and interpretation of circulating 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D in the clinical environment.

Authors: Bruce W Hollis
Journal: Endocrinol Metab Clin North Am Date: 2010-06 Impact factor: 4.741

A radioimmunoassay for 1,25-dihydroxycholecalciferol.

1. The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going.

Review 2. The clinical use of vitamin D metabolites and their potential developments: a position statement from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) and the International Osteoporosis Foundation (IOF).

3. Regulation of human tonsillar T-cell proliferation by the active metabolite of vitamin D3.

Review 4. Assessment and interpretation of circulating 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D in the clinical environment.

5. Metabolism of 25-hydroxyvitamin D3 by cultured pulmonary alveolar macrophages in sarcoidosis.

6. Serum concentrations of vitamin D metabolites in untreated tuberculosis.

7. Treatment of bone disease after jejunoileal bypass for obesity with oral 1 alpha-hydroxyvitamin D3.

8. Vitamin D Deficiency and Its Health Consequences in Africa.

9. There is more to life than serum vitamin D: a lesson from the past.