Literature DB >> 6307746

Dephosphorylation and reactivation of phosphorylated purified ox-kidney branched-chain dehydrogenase complex by co-purified phosphatase.

H R Fatania, P A Patston, P J Randle.   

Abstract

The branched-chain 2 oxoacid dehydrogenase complex has been purified from well-washed ox-kidney mitochondria together with branched-chain dehydrogenase kinase. The complex was inactivated and phosphorylated by ATP in about 5 min at 30 degrees C. After hydrolysis of ATP by a contaminating ATPase (5-10 min) the complex was dephosphorylated and reactivated. Dephosphorylation and reactivation were linearly correlated. Reactivation was dependent upon Mg2+ (K0.5 greater than 1 mM) and inhibited completely by 50 mM fluoride. Reactivation and dephosphorylation are attributed to a mitochondrial branched-chain dehydrogenase phosphatase.

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Year:  1983        PMID： 6307746     DOI： 10.1016/0014-5793(83)80585-7

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Regulation of the branched-chain 2-oxo acid dehydrogenase complex in hepatocytes isolated from rats fed on a low-protein diet.

Authors:  R A Harris; R Paxton; G W Goodwin; S M Powell
Journal:  Biochem J       Date:  1986-03-01       Impact factor: 3.857

2.  Purification and properties of a protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex.

Authors:  J Espinal; P A Patston; H R Fatania; K S Lau; P J Randle
Journal:  Biochem J       Date:  1985-01-15       Impact factor: 3.857

3.  Maple syrup urine disease caused by a partial deletion in the inner E2 core domain of the branched chain alpha-keto acid dehydrogenase complex due to aberrant splicing. A single base deletion at a 5'-splice donor site of an intron of the E2 gene disrupts the consensus sequence in this region.

Authors:  H Mitsubuchi; Y Nobukuni; I Akaboshi; Y Indo; F Endo; I Matsuda
Journal:  J Clin Invest       Date:  1991-04       Impact factor: 14.808

4.  The activity state of the branched-chain 2-oxo acid dehydrogenase complex in rat tissues.

Authors:  A J Wagenmakers; J T Schepens; J A Veldhuizen; J H Veerkamp
Journal:  Biochem J       Date:  1984-05-15       Impact factor: 3.857

5.  Maple syrup urine disease. Complete primary structure of the E1 beta subunit of human branched chain alpha-ketoacid dehydrogenase complex deduced from the nucleotide sequence and a gene analysis of patients with this disease.

Authors:  Y Nobukuni; H Mitsubuchi; F Endo; I Akaboshi; J Asaka; I Matsuda
Journal:  J Clin Invest       Date:  1990-07       Impact factor: 14.808

6.  Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.

Authors:  P J Sykes; G Burns; J Menard; K Hatter; J R Sokatch
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

7.  Purification and properties of branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney.

Authors:  Z Damuni; M L Merryfield; J S Humphreys; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  Effects of diet and of alloxan-diabetes on the activity of branched-chain 2-oxo acid dehydrogenase complex and of activator protein in rat tissues.

Authors:  P A Patston; J Espinal; P J Randle
Journal:  Biochem J       Date:  1984-09-15       Impact factor: 3.857

9.  Increase of the activity state and loss of total activity of the branched-chain 2-oxo acid dehydrogenase in rat diaphragm during incubation.

Authors:  A J Wagenmakers; J T Schepens; J H Veerkamp
Journal:  Biochem J       Date:  1984-12-01       Impact factor: 3.857
  9 in total

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