Subcellular distribution of mannophosphoinositides in Mycobacterium smegmatis during growth.

Subcellular distribution of total phospholipids and mannophosphoinositides (mannosides) was examined in Mycobacterium smegmatis ATCC 607 during its transition from the early exponential to the stationary phase of growth. There was relatively more of these substances in the cell membrane than in the cell wall, and the total amount increased with the age of the culture. Among individual mannosides, dimannophosphoinositides (dimannosides) were distributed equally in the cell wall and membrane. However, hexamannophosphoinositides (hexamannosides) were more predominant in the cell membrane, and the level increased with the age of the culture.