Literature DB >> 6307263

Pseudomonas cytochrome C-551 peroxidase. A purification procedure and study of CO-binding kinetics.

N Foote, A C Thompson, D Barber, C Greenwood.   

Abstract

A procedure is described for the purification of cytochrome c peroxidase from Pseudomonas aeruginosa involving extraction by sonication, followed by acid precipitation and chromatography on only two types of gel. The final preparation had a purity ratio A407/A280 of 4.2, and was found to be essentially pure by isoelectric focusing. The enzyme was shown to be unstable during degassing under vacuum except in the presence of detergent. The kinetics of CO binding to dithionite-reduced peroxidase were studied with stopped-flow and flash-photolysis techniques, and the results obtained between pH 5 and 7 suggest the existence of two forms of dithionite-reduced enzyme in slow equilibrium.

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Year:  1983        PMID： 6307263      PMCID： PMC1154148          DOI： 10.1042/bj2090701

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Pseudomonas cytochrome c peroxidase. X. The effect of Pseudomonas neutral proteinase on the enzyme molecule.

Authors:  R Soininen; N Ellfolk
Journal:  Acta Chem Scand B       Date:  1975

2.  Pseudomonas cytochrome c peroxidase. 8. The amino acid composition of the enzyme.

Authors:  R Soininen; N Ellfolk
Journal:  Acta Chem Scand       Date:  1973

3.  Pseudomonas cytochrome c peroxidase. VI. Large scale purification procedure.

Authors:  R Soininen
Journal:  Acta Chem Scand       Date:  1972

4.  Pseudomonas cytochrome c peroxidase. V. Absorption spectra of the enzyme and of its compounds with ligands. Inhibition of the enzyme by cyanide and azide.

Authors:  R Soininen; N Ellfolk
Journal:  Acta Chem Scand       Date:  1973

5.  Cytochrome c-556, a di-heme protein from Pseudomonas aeruginosa.

Authors:  J Singh; D C Wharton
Journal:  Biochim Biophys Acta       Date:  1973-02-22

6.  Pseudomonas cytochrome c peroxidase. 3. The size and shape of the enzyme molecule.

Authors:  N Ellfolk; R Soininen
Journal:  Acta Chem Scand       Date:  1971

7.  Pseudomonas cytochrome c peroxidase. I. Purification procedure.

Authors:  N Ellfolk; R Soininen
Journal:  Acta Chem Scand       Date:  1970

8.  Apparatus for rapid and sensitive spectrophotometry.

Authors:  Q H Gibson; L Milnes
Journal:  Biochem J       Date:  1964-04       Impact factor: 3.857

9.  A temperature-jump study of the reaction between azurin and cytochrome c oxidase from Pseudomonas aeruginosa.

Authors:  M Brunori; S R Parr; C Greenwood; M T Wilson
Journal:  Biochem J       Date:  1975-10       Impact factor: 3.857

10.  Pseudomonas cytochrome c peroxidase. II. Localization of cytochrome c peroxidase in Pseudomonas fluorescens.

Authors:  R Soininen; H Sojonen; N Ellfolk
Journal:  Acta Chem Scand       Date:  1970
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  9 in total

1.  A quantitative model for the mechanism of action of the cytochrome c peroxidase of Pseudomonas aeruginosa.

Authors:  N Foote; R Turner; T Brittain; C Greenwood
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

2.  Redox-linked spin-state changes in the di-haem cytochrome c-551 peroxidase from Pseudomonas aeruginosa.

Authors:  N Foote; J Peterson; P M Gadsby; C Greenwood; A J Thomson
Journal:  Biochem J       Date:  1985-08-15       Impact factor: 3.857

3.  Purification and properties of formate dehydrogenase from Pseudomonas aeruginosa. Characterization of haem and iron-sulphur centres by magnetic-circular-dichroism and electron-paramagnetic-resonance spectroscopy.

Authors:  C Godfrey; A Coddington; C Greenwood; A J Thomson; P M Gadsby
Journal:  Biochem J       Date:  1987-04-01       Impact factor: 3.857

4.  The cellular location and specificity of bacterial cytochrome c peroxidases.

Authors:  C F Goodhew; I B Wilson; D J Hunter; G W Pettigrew
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

5.  Analysis of the activation mechanism of Pseudomonas stutzeri cytochrome c peroxidase through an electron transfer chain.

Authors:  P M Paes de Sousa; D Rodrigues; C G Timóteo; M L Simões Gonçalves; G W Pettigrew; I Moura; J J G Moura; M M Correia dos Santos
Journal:  J Biol Inorg Chem       Date:  2011-05-06       Impact factor: 3.358

6.  Electrochemical evidence for multiple peroxidatic heme states of the diheme cytochrome c peroxidase of Pseudomonas aeruginosa.

Authors:  Clinton F Becker; Nicholas J Watmough; Sean J Elliott
Journal:  Biochemistry       Date:  2009-01-13       Impact factor: 3.162

7.  The nature of species prepared by photolysis of half-reduced, fully reduced and fully reduced carbonmonoxy-cytochrome c-551 peroxidase from Pseudomonas aeruginosa.

Authors:  C Greenwood; N Foote; J Peterson; A J Thomson
Journal:  Biochem J       Date:  1984-10-15       Impact factor: 3.857

8.  A study of the oxidized form of Pseudomonas aeruginosa cytochrome c-551 peroxidase with the use of magnetic circular dichroism.

Authors:  N Foote; J Peterson; P M Gadsby; C Greenwood; A J Thomson
Journal:  Biochem J       Date:  1984-10-15       Impact factor: 3.857

9.  The kinetics of the oxidation of cytochrome c by Paracoccus cytochrome c peroxidase.

Authors:  R Gilmour; C F Goodhew; G W Pettigrew; S Prazeres; J J Moura; I Moura
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857
  9 in total

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