Literature DB >> 630704

An enzymatic assay for determining free and total cholesterol in tissue.

J L De Hoff, L M Davidson, D Kritchevsky.   

Abstract

We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use of a cholesterol esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The lipids are solubilized (as micelles) directly into the aqueous reagent mixture. Results of the enzymatic method correlate well with those obtained by an acid/ferric chloride assay of digitonin precipitates of the extracts. The enzymatic assay of free and total cholesterol in tissue offers advantages of simplicity over previously used methods.

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Year:  1978        PMID： 630704

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  26 in total

1.  Hepatic very-low-density lipoprotein and apolipoprotein B production are increased following in vivo induction of betaine-homocysteine S-methyltransferase.

Authors:  Janet D Sparks; Heidi L Collins; Doru V Chirieac; Joanne Cianci; Jenny Jokinen; Mark P Sowden; Chad A Galloway; Charles E Sparks
Journal:  Biochem J       Date:  2006-04-15       Impact factor: 3.857

2.  The effect of dietary vitamin E supply and a moderately oxidized oil on activities of hepatic lipogenic enzymes in rats.

Authors:  K Eder; M Kirchgessner
Journal:  Lipids       Date:  1998-03       Impact factor: 1.880

3.  Effects of dietary thermoxidized fats on expression and activities of hepatic lipogenic enzymes in rats.

Authors:  Klaus Eder; Andrea Suelzle; Peter Skufca; Corinna Brandsch; Frank Hirche
Journal:  Lipids       Date:  2003-01       Impact factor: 1.880

4.  Impact of a high-protein diet during lactation on milk composition and offspring in a pig model.

Authors:  Alexandra Schutkowski; Holger Kluge; Paula Trotz; Gerd Hause; Bettina König; Monika Wensch-Dorendorf; Gabriele I Stangl
Journal:  Eur J Nutr       Date:  2018-11-28       Impact factor: 5.614

5.  Effect of pectin and cellulose on formation and regression of gallstones in hamsters.

Authors:  D Kritchevsky; S A Tepper; D M Klurfeld
Journal:  Experientia       Date:  1984-04-15

6.  Glutathione deficiency down-regulates hepatic lipogenesis in rats.

Authors:  Corinna Brandsch; Tobias Schmidt; Diana Behn; Kristin Weisse; Andreas S Mueller; Gabriele I Stangl
Journal:  Lipids Health Dis       Date:  2010-05-19       Impact factor: 3.876

7.  Changes in the activities of lipoprotein lipase and the lipogenic enzymes in tumor-bearing rats.

Authors:  S Lanza-Jacoby; E E Miller; F E Rosato
Journal:  Lipids       Date:  1982-12       Impact factor: 1.880

8.  Different degrees of moderate iron deficiency modulate lipid metabolism of rats.

Authors:  G I Stangl; M Kirchgessner
Journal:  Lipids       Date:  1998-09       Impact factor: 1.880

9.  Levels of polyunsaturated fatty acids in tissues from zinc-deficient rats fed a linseed oil diet.

Authors:  K Eder; M Kirchgessner
Journal:  Lipids       Date:  1994-12       Impact factor: 1.880

10.  Zinc deficiency and activities of lipogenic and glycolytic enzymes in liver of rats fed coconut oil or linseed oil.

Authors:  K Eder; M Kirchgessner
Journal:  Lipids       Date:  1995-01       Impact factor: 1.880
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