Literature DB >> 6306033

Purification of the group-specific antigen of bluetongue virus by chromatofocusing.

O J Hübschle, C Yang.   

Abstract

A method for the purification of the precipitating antigen of bluetongue virus (BTV) is described. The results obtained indicate that the precipitating antigen is identical with a core protein of BTV having a molecular weight of 39,000 (P7). So far all bluetongue virus serotypes have been shown to possess this protein. It is evident therefore that a group-specific reaction could be based on the presence of antibodies against this core protein. The purification of this protein by chromatofocusing proved relatively easy to perform and immunodiffusion tests revealed a group-specific reaction.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6306033     DOI： 10.1016/0166-0934(83)90029-0

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  2 in total

1.  Expression of two related nonstructural proteins of bluetongue virus (BTV) type 10 in insect cells by a recombinant baculovirus: production of polyclonal ascitic fluid and characterization of the gene product in BTV-infected BHK cells.

Authors:  T J French; S Inumaru; P Roy
Journal:  J Virol       Date:  1989-08       Impact factor: 5.103

2.  Vaccinia virus expression of the VP7 protein of South African bluetongue virus serotype 4 and its use as an antigen in a capture ELISA.

Authors:  M Cloete; D H du Plessis; A A van Dijk; H Huismans; G J Viljoen
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.