Literature DB >> 6306020

Delimitation of a DNA sequence which confers inducibility by glucocorticoid hormones.

B Groner, P Herrlich, N Kennedy, H Ponta, U Rahmsdorf, N E Hynes.   

Abstract

A chimeric long terminal repeat-thymidine kinase (LTR-tk) gene has been used to define the sequence requirements for glucocorticoid induction of gene expression. The original LTR-tk gene contains an entire mouse mammary tumor virus (MMTV) LTR preceding the tk gene. This gene can be expressed in a hormone-responsive fashion upon transfection into L tk--cells to produce a chimeric LTR-tk mRNA. Stepwise deletion of nucleotide sequences 5' of the viral RNA initiation site revealed that 202 nucleotides upstream of the viral cap site are sufficient for the hormonal regulation. Deletion of 5' sequences up to 59 nucleotides upstream of the viral cap site abolished RNA initiation in the LTR and hormonal induction.

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Year:  1982        PMID： 6306020     DOI： 10.1002/jcb.240200405

Source DB:  PubMed          Journal:  J Cell Biochem        ISSN： 0730-2312            Impact factor:   4.429


  4 in total

1.  Photocontrol of the Expression of Genes Encoding Chlorophyll a/b Binding Proteins and Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase in Etiolated Seedlings of Lycopersicon esculentum (L.) and Nicotiana tabacum (L.).

Authors:  B Wehmeyer; A R Cashmore; E Schäfer
Journal:  Plant Physiol       Date:  1990-07       Impact factor: 8.340

2.  Hormonal response region in the mouse mammary tumor virus long terminal repeat can be dissociated from the proviral promoter and has enhancer properties.

Authors:  H Ponta; N Kennedy; P Skroch; N E Hynes; B Groner
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

3.  Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs during fungus infection of soybean (Glycine max L.) roots or elicitor treatment of soybean cell cultures at the onset of phytoalexin synthesis.

Authors:  H Habereder; G Schröder; J Ebel
Journal:  Planta       Date:  1989-01       Impact factor: 4.116

4.  Fluence dependence of the ultraviolet-light-induced accumulation of chalcone synthase mRNA and effects of blue and far-red light in cultured parsley cells.

Authors:  B Bruns; K Hahlbrock; E Schäfer
Journal:  Planta       Date:  1986-11       Impact factor: 4.116
  4 in total

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