Literature DB >> 6306012

Poliovirus and polio antibody assay in HEp-2 and Vero cell cultures.

P Albrecht, J C Enterline, E J Boone, M J Klutch.   

Abstract

HEp-2 cell cultures were about three to 30 times more sensitive for poliovirus titration than Vero cells. Attenuated strains induced a complete cytopathic effect in HEp-2 but not in Vero cells. For polio antibody titration, HEp-2 and Vero cells were equally suitable. A high degree of sensitivity and reproducibility of virus neutralization was achieved in tests utilizing a low virus dose and serum-virus incubation overnight at 36 degrees C. Staining of infected trays with crystal violet obviated reading of viral CPE under the microscope and expedited the evaluation of larger-scale tests.

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Year:  1983        PMID： 6306012     DOI： 10.1016/s0092-1157(83)80031-6

Source DB:  PubMed          Journal:  J Biol Stand        ISSN： 0092-1157


  8 in total

1.  A mutation present in the amino terminus of Sabin 3 poliovirus VP1 protein is attenuating.

Authors:  J M Tatem; C Weeks-Levy; A Georgiu; S J DiMichele; E J Gorgacz; V R Racaniello; F R Cano; S J Mento
Journal:  J Virol       Date:  1992-05       Impact factor: 5.103

2.  Antiplasmodial activity of botanical extracts against Plasmodium falciparum.

Authors:  Asokan Bagavan; Abdul Abdul Rahuman; Chinnaperumal Kamaraj; Naveen Kumar Kaushik; Dinesh Mohanakrishnan; Dinkar Sahal
Journal:  Parasitol Res       Date:  2010-11-16       Impact factor: 2.289

3.  Evaluating associations between vaccine response and malnutrition, gut function, and enteric infections in the MAL-ED cohort study: methods and challenges.

Authors:  Christel Hoest; Jessica C Seidman; William Pan; Ramya Ambikapathi; Gagandeep Kang; Margaret Kosek; Stacey Knobler; Carl J Mason; Mark Miller
Journal:  Clin Infect Dis       Date:  2014-11-01       Impact factor: 9.079

4.  Immunogenicity and safety of combined adsorbed low-dose diphtheria, tetanus and inactivated poliovirus vaccine (REVAXIS (®)) versus combined diphtheria, tetanus and inactivated poliovirus vaccine (DT Polio (®)) given as a booster dose at 6 years of age.

Authors:  Vincent Gajdos; Benoit Soubeyrand; Emmanuel Vidor; Patrick Richard; Julie Boyer; Christine Sadorge; Anne Fiquet
Journal:  Hum Vaccin       Date:  2011-05-01

5.  Immunogenicity of sequential poliovirus vaccination schedules with different strains of poliomyelitis vaccines in Chongqing, China: a cross-sectional survey.

Authors:  Jiawei Xu; Qing Wang; Shanshan Kuang; Rong Rong; Yuanyuan Zhang; Xiaojuan Fu; Wenge Tang
Journal:  Hum Vaccin Immunother       Date:  2021-03-24       Impact factor: 3.452

6.  Antibody persistence following administration of a hexavalent DTwP-IPV-HB-PRP∼T vaccine versus separate DTwP-HB-PRP∼T and IPV vaccines at 12-24 months of age and safety and immunogenicity of a booster dose of DTwP-IPV-HB-PRP∼T in healthy infants in India.

Authors:  S Mangarule; S Palkar; M Mitra; M D Ravi; R Singh; A Moureau; M V Jayanth; D M Patel; S Ravinuthala; B N Patnaik; E Jordanov; F Noriega
Journal:  Vaccine X       Date:  2022-07-02

7.  Lot-to-lot consistency of a hexavalent DTwP-IPV-HB-PRP∼T vaccine and non-inferiority to separate DTwP-HB-PRP∼T and IPV antigen-matching vaccines at 6-8, 10-12, and 14-16 weeks of age co-administered with oral rotavirus vaccine in healthy infants in India: A multi-center, randomized, controlled study.

Authors:  S Mangarule; S Prashanth; A Kawade; M D Ravi; I V Padmavathi; S Palkar; V N Tripathi; R Singh; M Maurya; M Mitra; R S Shetty; R Z Kompithra; S M Dhaded; V Epari; A Moureau; M V Jayanth; K Varghese; S Ravinuthala; D Kukian; B N Patnaik; F Noriega
Journal:  Vaccine X       Date:  2022-09-13

8.  Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine.

Authors:  Hasmik Manukyan; Elvira Rodionova; Tatiana Zagorodnyaya; Tsai-Lien Lin; Konstantin Chumakov; Majid Laassri
Journal:  Virol J       Date:  2019-10-28       Impact factor: 4.099
  8 in total

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