Immunoreactive prolyl hydroxylase protein and galactosylhydroxylysyl glucosyltransferase activity in breast tissues and sera of patients with primary breast cancer.

Prolyl hydroxylase activity, immunoreactive prolyl hydroxylase protein and galactosylhydroxylysyl glucosyltransferase activity were determined in primary human breast cancer tissues and sera of the same patients, and compared to the level of enzyme activities and protein in normal breast tissues and control sera. The mean prolyl hydroxylase (PH) activity, B-IRPH protein, and B-GGT values in primary breast cancer tissues were 13.86, 5.28 and 5.00 times the mean levels of PH activity, B-IRPH protein and B-GGT in normal breast tissues, respectively. There was no difference in the levels of serum immunoreactive prolyl hydroxylase protein and serum galactosylhydroxylysyl glucosyltransferase activity in breast cancer patients and the controls. These results suggest that the prolyl hydroxylase activity, immunoreactive prolyl hydroxylase protein and galactosylhydroxylysyl glucosyltransferase activity may be elevated in primary breast cancer tissues to compensate for an increase rate of collagen synthesis.