Literature DB >> 6304005

Overproduction of Escherichia coli replication proteins by the use of runaway-replication plasmids.

S Yasuda, T Takagi.   

Abstract

A derivative of the runaway-replication plasmid was constructed. This plasmid, pSY343, has the gene for kanamycin resistance and single sites for EcoRI, BamHI, HindIII, KpnI, and XhoI that can be used as cloning sites without inactivating the kanamycin resistance gene or the replication genes. Three replication genes of Escherichia coli were cloned on the plasmid. The activity of dnaA, dnaZ, and ssb gene products were 200-, 90-, and 60-fold greater, respectively, in the cells containing these plasmids than in normal cells.

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Year:  1983        PMID： 6304005      PMCID： PMC217586          DOI： 10.1128/jb.154.3.1153-1161.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  28 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  DNA polymerase III holoenzyme of Escherichia coli. Purification and resolution into subunits.

Authors:  C McHenry; A Kornberg
Journal:  J Biol Chem       Date:  1977-09-25       Impact factor: 5.157

3.  Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli.

Authors:  C C Filip; J S Allen; R A Gustafson; R G Allen; J R Walker
Journal:  J Bacteriol       Date:  1974-08       Impact factor: 3.490

4.  The construction in vitro of transducing derivatives of phage lambda.

Authors:  K Borck; J D Beggs; W J Brammar; A S Hopkins; N E Murray
Journal:  Mol Gen Genet       Date:  1976-07-23

5.  A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors:  L Clarke; J Carbon
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

6.  Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro.

Authors:  S Wickner; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

7.  Characterization of a mini-ColC1 plasmid.

Authors:  V Hershfield; H W Boyer; L Chow; D R Helinski
Journal:  J Bacteriol       Date:  1976-04       Impact factor: 3.490

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Cloning and mapping of the replication origin of Escherichia coli.

Authors:  S Yasuda; Y Hirota
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity.

Authors:  M Matsuhashi; Y Takagaki; I N Maruyama; S Tamaki; Y Nishimura; H Suzuki; U Ogino; Y Hirota
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205
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  22 in total

1.  The Escherichia coli polB gene, which encodes DNA polymerase II, is regulated by the SOS system.

Authors:  H Iwasaki; A Nakata; G C Walker; H Shinagawa
Journal:  J Bacteriol       Date:  1990-11       Impact factor: 3.490

2.  Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay.

Authors:  A Anastasi; C G Knight; A J Barrett
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

3.  Posttranscriptional repression of Escherichia coli OmpF protein in response to redox stress: positive control of the micF antisense RNA by the soxRS locus.

Authors:  J H Chou; J T Greenberg; B Demple
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

4.  Interaction of RecBCD enzyme with DNA at double-strand breaks produced in UV-irradiated Escherichia coli: requirement for DNA end processing.

Authors:  B Thoms; W Wackernagel
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

5.  Escherichia coli parA is an allele of the gyrB gene.

Authors:  J Kato; Y Nishimura; H Suzuki
Journal:  Mol Gen Genet       Date:  1989-05

Review 6.  DNA-protein interactions during replication of genetic elements of bacteria.

Authors:  J Nesvera; J Hochmannová
Journal:  Folia Microbiol (Praha)       Date:  1985       Impact factor: 2.099

7.  Escherichia coli DnaX product, the tau subunit of DNA polymerase III, is a multifunctional protein with single-stranded DNA-dependent ATPase activity.

Authors:  S H Lee; J R Walker
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

8.  Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.

Authors:  J E Rutila; M J Imperiale; W W Brockman
Journal:  J Virol       Date:  1986-05       Impact factor: 5.103

9.  Preparation of the FhuA (TonA) receptor protein from cell envelopes of an overproducing strain of Escherichia coli K-12.

Authors:  H Hoffmann; E Fischer; H Kraut; V Braun
Journal:  J Bacteriol       Date:  1986-05       Impact factor: 3.490

10.  Complete nucleotide sequence of the Escherichia coli recC gene and of the thyA-recC intergenic region.

Authors:  P W Finch; R E Wilson; K Brown; I D Hickson; A E Tomkinson; P T Emmerson
Journal:  Nucleic Acids Res       Date:  1986-06-11       Impact factor: 16.971
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