Pulmonary alpha-adrenoceptors: autoradiographic localization using [3H]prazosin.

We determined the distribution of pulmonary alpha-adrenoceptors by autoradiographic localisation of [3H]prazosin binding to frozen sections of ferret lung. Specific binding of [3H]prazosin to lung sections was saturable and of high affinity (KD = 0.44 +/- 0.55 nM; mean +/- S.E., n = 5), with a specificity indicating binding to alpha 1-receptors. Autoradiographic showed that alpha 1-receptors were present in highest density in vascular smooth muscle (small vessels greater than large vessels), and were also present in airway submucosal glands and epithelium. There was also scanty labelling of alveolar walls which may be to contractile interstitial cells (Kapanci cells). Although smooth muscle of bronchi showed little labelling, surprisingly that of bronchioles was heavily labelled. The high density of alpha-receptors in small airways may be relevant to asthma in which alpha-adrenergic responses are activated. This method offers a means by which autonomic receptors of small airways may be investigated without the confusing contribution of other contractile elements and larger airways.