Literature DB >> 6303158

Direct hybridization of labeled DNA to DNA in agarose gels.

M Purrello, I Balazs.   

Abstract

A fast and simple procedure for the hybridization of radioactive probes directly to DNA in agarose gels is described. Restriction endonuclease-digested DNA is fractionated by electrophoresis in agarose gel. After drying, the DNA in the gel is denatured with alkali and annealed with a radioactive probe, and the hybridization pattern visualized by autoradiography. This method is sensitive enough to detect single or low copy number DNA sequences in the genome of higher eucaryotes.

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Year:  1983        PMID： 6303158     DOI： 10.1016/0003-2697(83)90391-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  30 in total

1.  Preparation of DNA and protein micro arrays on glass slides coated with an agarose film.

Authors:  V Afanassiev; V Hanemann; S Wölfl
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

2.  A rapid method of non-radioactive detection of DNA-fragments in dried agarose gels.

Authors:  L Yavachev
Journal:  Nucleic Acids Res       Date:  1991-01-11       Impact factor: 16.971

3.  Conjugal strategy for construction of fast Acid-producing, bacteriophage-resistant lactic streptococci for use in dairy fermentations.

Authors:  M E Sanders; P J Leonhard; W D Sing; T R Klaenhammer
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

4.  Integrative Cloning, Expression, and Stability of the cryIA(c) Gene from Bacillus thuringiensis subsp. kurstaki in a Recombinant Strain of Clavibacter xyli subsp. cynodontis.

Authors:  J S Lampel; G L Canter; M B Dimock; J L Kelly; J J Anderson; B B Uratani; J S Foulke; J T Turner
Journal:  Appl Environ Microbiol       Date:  1994-02       Impact factor: 4.792

5.  Vector-Alu PCR: a rapid step in mapping cosmids and YACs.

Authors:  C Breukel; J Wijnen; C Tops; H vd Klift; H Dauwerse; P M Khan
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

6.  Activation of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) lytic replication by human cytomegalovirus.

Authors:  J Vieira; P O'Hearn; L Kimball; B Chandran; L Corey
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

7.  New class of limited-host-range Agrobacterium mega-tumor-inducing plasmids lacking homology to the transferred DNA of a wide-host-range, tumor-inducing plasmid.

Authors:  L Unger; S F Ziegler; G A Huffman; V C Knauf; R Peet; L W Moore; M P Gordon; E W Nester
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

8.  Genetic structure and domains of DNA polymerase III of Bacillus subtilis.

Authors:  B Sanjanwala; A T Ganesan
Journal:  Mol Gen Genet       Date:  1991-05

9.  A human autosomal phosphoglycerate kinase locus maps near the HLA cluster.

Authors:  P Szabo; K H Grzeschik; M Siniscalco
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

10.  Old and new genetics help ordering loci at the telomere of the human X-chromosome long arm.

Authors:  M Purrello; R Nussbaum; A Rinaldi; G Filippi; S Traccis; B Latte; M Siniscalco
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132
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