Literature DB >> 6302982

Molecular biology of rotaviruses. V. Terminal structure of viral RNA species.

M A McCrae, J G McCorquodale.   

Abstract

The terminal structure of rotavirus genomic ds RNA and in vitro transcribed mRNA was investigated using S1 nuclease digestion and 3' terminal sequence analysis. S1 nuclease analysis indicated that viral mRNA was not a truncated copy of the genome RNA at its 5' end. The 3' terminal sequence analysis of genomic plus strands and mRNA showed that they were coterminal indicating that in vitro transcribed mRNA is a full length copy of the genomic template. The sequence analysis performed on isolated viral RNA species plus strands revealed a completely conserved 3' terminal octanucleotide 5'-AUGUGACC-3' present in both UK tissue culture adapted bovine rotavirus and a human rotavirus isolate. Sequencing of isolated genomic minus strands again revealed a completely conserved 3' terminal sequence of approximately the same length as that seen for the viral plus strands.

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Year:  1983        PMID： 6302982     DOI： 10.1016/0042-6822(83)90472-5

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  29 in total

1.  A four-nucleotide translation enhancer in the 3'-terminal consensus sequence of the nonpolyadenylated mRNAs of rotavirus.

Authors:  V Chizhikov; J T Patton
Journal:  RNA       Date:  2000-06       Impact factor: 4.942

2.  Identification and characterization of a transcription pause site in rotavirus.

Authors:  J A Lawton; M K Estes; B V Prasad
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

3.  Intracellular amplification and expression of a synthetic analog of rotavirus genomic RNA bearing a foreign marker gene: mapping cis-acting nucleotides in the 3'-noncoding region.

Authors:  M I Gorziglia; P L Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-01       Impact factor: 11.205

4.  Effect of intragenic rearrangement and changes in the 3' consensus sequence on NSP1 expression and rotavirus replication.

Authors:  J T Patton; Z Taraporewala; D Chen; V Chizhikov; M Jones; A Elhelu; M Collins; K Kearney; M Wagner; Y Hoshino; V Gouvea
Journal:  J Virol       Date:  2001-03       Impact factor: 5.103

5.  Synthesis of plus- and minus-strand RNA in rotavirus-infected cells.

Authors:  S Stacy-Phipps; J T Patton
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

6.  Reverse genetics system for introduction of site-specific mutations into the double-stranded RNA genome of infectious rotavirus.

Authors:  Satoshi Komoto; Jun Sasaki; Koki Taniguchi
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-14       Impact factor: 11.205

7.  Crystallographic and biochemical analysis of rotavirus NSP2 with nucleotides reveals a nucleoside diphosphate kinase-like activity.

Authors:  Mukesh Kumar; Hariharan Jayaram; Rodrigo Vasquez-Del Carpio; Xiaofang Jiang; Zenobia F Taraporewala; Raymond H Jacobson; John T Patton; B V Venkataram Prasad
Journal:  J Virol       Date:  2007-09-05       Impact factor: 5.103

8.  cis-Acting signals that promote genome replication in rotavirus mRNA.

Authors:  J T Patton; M Wentz; J Xiaobo; R F Ramig
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103

9.  RNA-binding and capping activities of proteins in rotavirus open cores.

Authors:  J T Patton; D Chen
Journal:  J Virol       Date:  1999-02       Impact factor: 5.103

10.  A Point Mutation in the Rhesus Rotavirus VP4 Protein Generated through a Rotavirus Reverse Genetics System Attenuates Biliary Atresia in the Murine Model.

Authors:  Sujit K Mohanty; Bryan Donnelly; Phylicia Dupree; Inna Lobeck; Sarah Mowery; Jaroslaw Meller; Monica McNeal; Greg Tiao
Journal:  J Virol       Date:  2017-07-12       Impact factor: 5.103
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