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Literature DB >> 6302132

Simplified method for typing herpes simplex virus by restriction endonuclease analysis.

Abstract

We have developed a simplified method for unambiguously typing herpes simplex virus. The method depends on the production of cell-associated virus at 34 degrees C and subsequently, on the separation of cellular DNA and viral DNA by Dounce homogenization and the removal of nuclei by centrifugation. Viral nucleic acid was prepared from the cytoplasmic fraction and analyzed after restriction endonuclease cleavage. The method obviates the use of radioactive isotopes, and the viral DNA is effectively free of interfering cellular DNA.

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Year: 1983 PMID： 6302132 PMCID： PMC272683 DOI： 10.1128/jcm.17.3.548-551.1983

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

16 in total

1. The effect of the temperature of incubation on the formation and release of herpes simplex virus in infected FL cells.

Authors: M D HOGGAN; B ROIZMAN
Journal: Virology Date: 1959-08 Impact factor: 3.616

2. Differential action of deoxynucleosides on mammalian cell cultures infected with herpes simplex virus types 1 and 2.

Authors: A D Kelman; F E Capozza; S Kibrick
Journal: J Infect Dis Date: 1975-04 Impact factor: 5.226

3. A new method for the isolation of herpes simplex virus type 2 DNA.

Authors: J M Walboomers; J T Schegget
Journal: Virology Date: 1976-10-01 Impact factor: 3.616

4. Efficiency of plating on chick embryo cells and kinetic neutralization of Herpesvirus hominis strains.

Authors: B B Wentworth; S L Zablotney
Journal: Infect Immun Date: 1972-03 Impact factor: 3.441

5. Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescent technique.

Authors: L Geder; G R Skinner
Journal: J Gen Virol Date: 1971-08 Impact factor: 3.891

6. Rapid identification and typing of herpes simplex virus types 1 and 2 by a direct immunofluorescence technique.

Authors: A Nahmias; I DelBuono; J Pipkin; R Hutton; C Wickliffe
Journal: Appl Microbiol Date: 1971-09

7. Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method.

Authors: D R Benjamin
Journal: Appl Microbiol Date: 1974-10

8. Typing of Herpesvirus hominis strains by a direct immunofluorescent technique.

Authors: A J Nahmias; W T Chiang; I Del Buono; A Duffey
Journal: Proc Soc Exp Biol Med Date: 1969-10

9. Use of biological characteristics to type Herpesvirus hominis types 1 and 2 in diagnostic laboratories.

Authors: S Marks-Hellman; M Ho
Journal: J Clin Microbiol Date: 1976-03 Impact factor: 5.948

10. Inhibition of herpes simplex virus type 2 replication by thymidine.

Authors: G H Cohen; M N Factor; M Ponce de Leon
Journal: J Virol Date: 1974-07 Impact factor: 5.103

7 in total

1. Improved DNA hybridization method for detection of acyclovir-resistant herpes simplex virus.

Authors: E M Swierkosz; D R Scholl; J L Brown; J D Jollick; C A Gleaves
Journal: Antimicrob Agents Chemother Date: 1987-10 Impact factor: 5.191

2. Problems associated with the use of (E)-5-(2-bromovinyl)-2'-deoxyuridine for typing herpes simplex virus.

Authors: E M Swierkosz; M Q Arens; K A Rivetna
Journal: J Clin Microbiol Date: 1985-03 Impact factor: 5.948

3. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.

Authors: C A Gleaves; D J Wilson; A D Wold; T F Smith
Journal: J Clin Microbiol Date: 1985-01 Impact factor: 5.948

Review 7. Herpes simplex virus infections.

Authors: R C Reichman
Journal: Eur J Clin Microbiol Date: 1984-10 Impact factor: 3.267