Literature DB >> 6301457

Polymerization of vector DNA after transfection into hamster fibroblast cells.

M L Wang, A S Lee.   

Abstract

We have transformed hamsters lung fibroblasts with two cloned, selectable markers, the widely used pSV2gpt vector and pNEO3, which confers resistance to neomycin. Analysis of DNA extracted from both individual and pooled groups of several hundred transformants reveal that in these carrier DNA-free transformation experiments, the vector DNA are found in concatenate forms in the transformants. The copy number of the integrated vectors can vary from a few to several hundred. In addition, the efficiency of transformation is ten times higher in the case of pSV2gpt.

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Year:  1983        PMID： 6301457     DOI： 10.1016/0006-291x(83)91191-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  11 in total

1.  Identification of a 70-base-pair cell cycle regulatory unit within the promoter of the human thymidine kinase gene and its interaction with cellular factors.

Authors:  Y K Kim; A S Lee
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

2.  A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties.

Authors:  A Y Lin; S C Chang; A S Lee
Journal:  Mol Cell Biol       Date:  1986-04       Impact factor: 4.272

3.  Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes.

Authors:  Y K Kim; S Wells; Y F Lau; A S Lee
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

4.  Transcriptional activation of the glucose-regulated protein genes and their heterologous fusion genes by beta-mercaptoethanol.

Authors:  Y K Kim; A S Lee
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

5.  Induction of two genes by glucose starvation in hamster fibroblasts.

Authors:  A Y Lin; A S Lee
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

6.  Identification of highly conserved regulatory domains and protein-binding sites in the promoters of the rat and human genes encoding the stress-inducible 78-kilodalton glucose-regulated protein.

Authors:  E Resendez; S K Wooden; A S Lee
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

7.  Rat gene encoding the 78-kDa glucose-regulated protein GRP78: its regulatory sequences and the effect of protein glycosylation on its expression.

Authors:  S C Chang; S K Wooden; T Nakaki; Y K Kim; A Y Lin; L Kung; J W Attenello; A S Lee
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

8.  Calcium ionophore A23187 induces expression of glucose-regulated genes and their heterologous fusion genes.

Authors:  E Resendez; J W Attenello; A Grafsky; C S Chang; A S Lee
Journal:  Mol Cell Biol       Date:  1985-06       Impact factor: 4.272

9.  Quantification of expression of linked cloned genes in a simian virus 40-transformed xeroderma pigmentosum cell line.

Authors:  M Protić-Sabljić; D Whyte; J Fagan; B H Howard; C M Gorman; R Padmanabhan; K H Kraemer
Journal:  Mol Cell Biol       Date:  1985-07       Impact factor: 4.272

10.  Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes.

Authors:  Stéphan Maurel; Marylène Mougel
Journal:  Retrovirology       Date:  2010-08-05       Impact factor: 4.602
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