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Literature DB >> 6301012

High-efficiency ligation and recombination of DNA fragments by vertebrate cells.

Abstract

DNA-mediated gene transfer (transfection) is used to introduce specific genes into vertebrate cells. Events soon after transfection were quantitatively analyzed by determining the infectivity of the DNA from an avian retrovirus and of mixtures of subgenomic fragments of this DNA. The limiting step of transfection with two DNA molecules is the uptake by a single cell of both DNA's in a biologically active state. Transfected cells mediate ligation and recombination of physically unlinked DNA's at nearly 100 percent efficiency.

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Year: 1983 PMID： 6301012 DOI： 10.1126/science.6301012

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

65 in total

1. Generation of hybrid human immunodeficiency virus utilizing the cotransfection method and analysis of cellular tropism.

Authors: A Velpandi; T Nagashunmugam; S Murthy; M Cartas; C Monken; A Srinivasan
Journal: J Virol Date: 1991-09 Impact factor: 5.103

2. Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells.

Authors: J S Mudgett; W D Taylor
Journal: Mol Cell Biol Date: 1990-01 Impact factor: 4.272

3. Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization.

Authors: G W Bates; S A Carle; W C Piastuch
Journal: Plant Mol Biol Date: 1990-06 Impact factor: 4.076

4. N myristoylation of the spleen necrosis virus matrix protein is required for correct association of the Gag polyprotein with intracellular membranes and for particle formation.

Authors: T A Weaver; A T Panganiban
Journal: J Virol Date: 1990-08 Impact factor: 5.103

10. Expression of a nuclear and a cytoplasmic Epstein-Barr virus early antigen after DNA transfer: cooperation of two distant parts of the genome for expression of the cytoplasmic antigen.

Authors: K Takaki; A Polack; G W Bornkamm
Journal: Proc Natl Acad Sci U S A Date: 1984-07 Impact factor: 11.205

High-efficiency ligation and recombination of DNA fragments by vertebrate cells.

1. Generation of hybrid human immunodeficiency virus utilizing the cotransfection method and analysis of cellular tropism.

2. Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells.

3. Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization.

4. N myristoylation of the spleen necrosis virus matrix protein is required for correct association of the Gag polyprotein with intracellular membranes and for particle formation.

5. Circularized Ac/Ds transposons: formation, structure and fate.

6. Positive genetic selection for gene disruption in mammalian cells by homologous recombination.

7. Gene recombination in X-ray-sensitive hamster cells.

8. Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.

9. Sequence homology requirements for intermolecular recombination in mammalian cells.

10. Expression of a nuclear and a cytoplasmic Epstein-Barr virus early antigen after DNA transfer: cooperation of two distant parts of the genome for expression of the cytoplasmic antigen.