Literature DB >> 630040

Quantum yield and image contrast of bacteriochlorophyll monolayers in photoelectron microscopy.

R B Barnes, J Amend, W R Sistrom, O H Griffith.   

Abstract

The photoelectron quantum yield spectrum of bacteriochlorophyll aGg (Bchl a ) from Rhodospirillum rubrum was determined in order to evaluate the possibility of mapping photoreceptor distribution and organization in bacterial chromatophores. The quantum yield is on the order of 1 X 10(-3) electrons/incident photon at 180 nm and decreases to 2.5 X 10(-5) electrons/incident photon at 230 nm. Photoelectron micrographs confirm the high contrast predicted between monolayers of Bchl a against a lipid background (calcium arachidate). A significant contrast difference is found between the two monolayer orientations, demonstrating that photoelectron microscopy is a sensitive detector of asymmetry in Bch1 a monolayers.

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Year:  1978        PMID: 630040      PMCID: PMC1473685          DOI: 10.1016/S0006-3495(78)85519-2

Source DB:  PubMed          Journal:  Biophys J        ISSN: 0006-3495            Impact factor:   4.033


  8 in total

1.  Some mutational changes in the photosynthetic pigment system of Rhodopseudomonas spheroides.

Authors:  M GRIFFITHS; R Y STANIER
Journal:  J Gen Microbiol       Date:  1956-07

2.  Photoelectron quantum yields and photoelectron microscopy of chlorophyll and chlorophyllin.

Authors:  R J Dam; K F Kongslie; O H Griffith
Journal:  Photochem Photobiol       Date:  1975-12       Impact factor: 3.421

3.  Relations between pigments and proteins in the photosynthetic membranes of Rhodopseudomonas spheroides.

Authors:  R K Clayton; B J Clayton
Journal:  Biochim Biophys Acta       Date:  1972-12-14

4.  Esterifying alcohols in the chlorophylls of purple photosynthetic bacteria. A new chlorophyll, bacteriochlorophyll (gg), all-trans-geranylgeranyl bacteriochlorophyllide a.

Authors:  J J Katz; H H Strain; A L Harkness; M H Studier; W A Svec; T R Janson; B T Cope
Journal:  J Am Chem Soc       Date:  1972-11-01       Impact factor: 15.419

5.  Complete fractionation of bacteriochlorophyll and its degradation products.

Authors:  W S Kim
Journal:  Bibl Laeger       Date:  1966-03-14

6.  The spectrum of bacteriochlorophyll in vivo: observations on mutants of Rhodopseudomonas spheroides unable to grow photosynthetically.

Authors:  W R Sistrom
Journal:  Photochem Photobiol       Date:  1966 Nov-Dec       Impact factor: 3.421

7.  Photoelectron quantum yields of the amino acids.

Authors:  R J Dam; C A Burke; O H Griffith
Journal:  Biophys J       Date:  1974-06       Impact factor: 4.033

8.  Photoelectron quantum yields of hemin, hemoglobin, and apohemoglobin. Possible applications to photoelectron microscopy of heme proteins in biological membranes.

Authors:  R J Dam; K F Kongslie; O H Griffith
Journal:  Biophys J       Date:  1974-12       Impact factor: 4.033

  8 in total
  2 in total

1.  Photoelectric properties and detection of the aromatic carcinogens benza[a]pyrene and dimethylbenzanthracene.

Authors:  W A Houle; H M Brown; O H Griffith
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Photoelectron microscopy and immunofluorescence microscopy of cytoskeletal elements in the same cells.

Authors:  K K Nadakavukaren; L B Chen; D L Habliston; O H Griffith
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

  2 in total

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