Literature DB >> 6300156

Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukaemia virus.

D Portetelle, C Bruck, M Mammerickx, A Burny.   

Abstract

A variant of the ELISA technique, involving a monoclonal anti-gp51 antibody yields a highly sensitive method for the detection of bovine leukaemia virus (BLV) antibodies. The gp51 antigen-coated microtitre plates are obtained by incubation of plastic-adsorbed monoclonal antibodies with a non-purified mixture of BLV antigens. Sera to be tested are incubated in the wells of the gp51-coated plates and bound antibodies are revealed by an enzyme-linked antibovine immunoglobulin reagent. This test is as sensitive as liquid phase radioimmunoassay using the same gp51 antigen and thus appears as a highly sensitive, practical, rapid and cheap method for the detection of BLV antibodies.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6300156     DOI： 10.1016/0166-0934(83)90064-2

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  6 in total

1.  Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to bovine leukemia virus in serum and milk.

Authors:  V K Nguyen; R F Maes
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

2.  Purification by Strep-Tactin affinity chromatography of a delete envelope gp51 protein of Bovine Leukaemia virus expressed in Sf21 insect cells.

Authors:  Antonio De Giuseppe; Katia Forti; Francesco Feliziani; Giulio Severi; Monica Cagiola
Journal:  Protein J       Date:  2010-04       Impact factor: 2.371

3.  Expression of the bovine leukemia virus envelope glycoprotein (gp51) by recombinant baculovirus and its use in an enzyme-linked immunosorbent assay.

Authors:  Antonio De Giuseppe; Francesco Feliziani; Domenico Rutili; Gian Mario De Mia
Journal:  Clin Diagn Lab Immunol       Date:  2004-01

4.  Early detection of bovine leukemia virus by using an enzyme-linked assay for polymerase chain reaction-amplified proviral DNA in experimentally infected cattle.

Authors:  H M Naif; R C Daniel; W G Cougle; M F Lavin
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

5.  Ultrastructural demonstration of retrovirus antigens with immuno-gold staining in prodromal acquired immune deficiency syndrome.

Authors:  W W Feremans; R Menu; M Goldman; L Schandene; D Portetelle; Y Cleuter; J Flament; P Dustin; J Wybran; A Burny
Journal:  J Clin Pathol       Date:  1984-12       Impact factor: 3.411

6.  Production, Characterization, and Use of Monoclonal Antibodies Against gp51 Protein to Diagnose Bovine Leukemia Virus Infection.

Authors:  Ludmilla D C Troiano; Vanete Thomaz-Soccol; Jorge V B Agottani; Josiane Brodzinski; Tania R Penha; Silvia C Ozaki
Journal:  Biores Open Access       Date:  2013-02
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.