Literature DB >> 6299865

An improved method for the isolation of islets from the beef pancreas.

P E Lacy, E T Lacy, E H Finke, Y Yasunami.   

Abstract

An improved method for the isolation of islets from the beef and pig pancreas is described. The procedure involves the use of strips of Velcro that retain the partially-digested collagen during the isolation of islets by the collagenase technique. The spiny portion of the Velcro is ideally suited to retain the collagen and yet permit the separation of islets from the pancreatic parenchyma. A remarkably high yield of islets has been obtained from the beef and pig pancreas using this procedure.

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Year:  1982        PMID: 6299865     DOI: 10.2337/diab.31.4.s109

Source DB:  PubMed          Journal:  Diabetes        ISSN: 0012-1797            Impact factor:   9.461


  9 in total

Review 1.  Culture of pancreatic islet cells and islet hormone producing cell lines "morphological and functional integrity in culture".

Authors:  R Takaki
Journal:  In Vitro Cell Dev Biol       Date:  1989-09

Review 2.  Prospects for pancreatic islet transplantation.

Authors:  D W Gray; P J Morris
Journal:  World J Surg       Date:  1986-06       Impact factor: 3.352

3.  Long-term survival and function of isolated bovine pancreatic islets maintained in different culture media.

Authors:  A Coppelli; C Arvia; R Giannarelli; P Marchetti; P Viacava; A G Naccarato; M Lorenzetti; S Cosimi; P Cecchetti; R Navalesi
Journal:  Acta Diabetol       Date:  1996-07       Impact factor: 4.280

Review 4.  The mechanism of insulin secretion.

Authors:  S L Howell
Journal:  Diabetologia       Date:  1984-05       Impact factor: 10.122

5.  Experimental immuno-alteration.

Authors:  P E Lacy
Journal:  World J Surg       Date:  1984-04       Impact factor: 3.352

Review 6.  Isolation and transplantation of islet tissue.

Authors:  D W Scharp
Journal:  World J Surg       Date:  1984-04       Impact factor: 3.352

7.  Presentation and outcome of rhino-orbital-cerebral mucormycosis in patients with diabetes.

Authors:  A Bhansali; S Bhadada; A Sharma; V Suresh; A Gupta; P Singh; A Chakarbarti; R J Dash
Journal:  Postgrad Med J       Date:  2004-11       Impact factor: 2.401

8.  Demonstration of donor specific unresponsiveness in rat islet allografts: importance of transplant site for induction by cyclosporin A and maintenance.

Authors:  T Kamei; Y Yasunami
Journal:  Diabetologia       Date:  1989-11       Impact factor: 10.122

9.  Pancreatic islet transplantation--experimental experience and clinical potential.

Authors:  K J Lafferty
Journal:  West J Med       Date:  1985-12
  9 in total

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