Literature DB >> 6299359

Evidence for extensive methylation of ribosomal RNA genes in a rat XC cell line.

R A Katz, B F Erlanger, R V Guntaka.   

Abstract

XC cell line was established from a rat fibrosarcoma induced by avian sarcoma virus. Analysis of ribosomal RNA genes in the DNA of this cell line indicated that there was a 3- to 4-fold increase in the number of the rDNA repeating units. [methyl-14C]Methionine labeling experiments as well as Southern blot analysis of the restriction endonuclease fragments showed extensive methylation of the cytosine residues in the rDNA. Further analysis by HpaII, HhaI and MspI suggested that the entire rDNA repeat unit was uniformly methylated in the recognition sequence for these enzymes. Parallel experiments with two other rat cell lines (rat embryo fibroblasts and adenovirus-transformed rat embryo cells) indicated no evidence of methylation in the rDNA.

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Year:  1983        PMID： 6299359     DOI： 10.1016/0167-4781(83)90099-4

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  12 in total

1.  Calcineurin regulates slow myosin, but not fast myosin or metabolic enzymes, during fast-to-slow transformation in rabbit skeletal muscle cell culture.

Authors:  J D Meissner; G Gros; R J Scheibe; M Scholz; H P Kubis
Journal:  J Physiol       Date:  2001-05-15       Impact factor: 5.182

2.  Fast-to-slow transformation and nuclear import/export kinetics of the transcription factor NFATc1 during electrostimulation of rabbit muscle cells in culture.

Authors:  Hans-Peter Kubis; Renate J Scheibe; Joachim D Meissner; Gunther Hornung; Gerolf Gros
Journal:  J Physiol       Date:  2002-06-15       Impact factor: 5.182

3.  GAPDH acts as an inducible not constitutive gene in cultured endothelial cells.

Authors:  J A Rimarachin; J Norcross; P Szabo; B B Weksler
Journal:  In Vitro Cell Dev Biol       Date:  1992 Nov-Dec

4.  Human cyclophilin 40 is a heat shock protein that exhibits altered intracellular localization following heat shock.

Authors:  P J Mark; B K Ward; P Kumar; H Lahooti; R F Minchin; T Ratajczak
Journal:  Cell Stress Chaperones       Date:  2001-01       Impact factor: 3.667

5.  Regulation of the Hsp90-binding immunophilin, cyclophilin 40, is mediated by multiple sites for GA-binding protein (GABP).

Authors:  P Kumar; B K Ward; R F Minchin; T Ratajczak
Journal:  Cell Stress Chaperones       Date:  2001-01       Impact factor: 3.667

6.  Retinoic acid modulates rat Ito cell proliferation, collagen, and transforming growth factor beta production.

Authors:  B H Davis; R T Kramer; N O Davidson
Journal:  J Clin Invest       Date:  1990-12       Impact factor: 14.808

7.  Growth hormone-releasing factor regulates growth hormone mRNA in primary cultures of rat pituitary cells.

Authors:  G G Gick; F N Zeytin; P Brazeau; N C Ling; F S Esch; C Bancroft
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

8.  5-Methyldeoxycytidine in the Physarum minichromosome containing the ribosomal RNA genes.

Authors:  C A Cooney; H R Matthews; E M Bradbury
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

9.  Mutations in the promoter region of the gene for gp91-phox in X-linked chronic granulomatous disease with decreased expression of cytochrome b558.

Authors:  P E Newburger; D G Skalnik; P J Hopkins; E A Eklund; J T Curnutte
Journal:  J Clin Invest       Date:  1994-09       Impact factor: 14.808

10.  Ontogeny of the secretory immune system: maturation of a functional polymeric immunoglobulin receptor regulated by gene expression.

Authors:  S Huling; G R Fournier; A Feren; A Chuntharapai; A L Jones
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205
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