Literature DB >> 629891

A simplified method for rapid determination of porphyrins by thin-layer chromatography.

T Okuda, H Nakajima, K Yatsuki, M Amano, G Umeda.   

Abstract

Free porphyrins, especially copro-, uro-, and protoporphyrin, were successfully separated by talc thin-layer chromatography (TLC), and were determined from the relationship of the weight and the area of porphyrins in the spot on the thin-layer plate. The results obtained from both the solvent extraction and the simple, rapid TLC method reported in this paper showed good correlation for coproporphyrin levels up to 400 microgram/1. The TLC method was applied to the determination of urinary coproporphyrin in lead workers and in rats.

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Year:  1978        PMID: 629891      PMCID: PMC1008326          DOI: 10.1136/oem.35.1.61

Source DB:  PubMed          Journal:  Br J Ind Med        ISSN: 0007-1072


  6 in total

1.  Mitochondrial coproporphyrinogen oxidase and protoporphyrin formation.

Authors:  S SANO; S GRANICK
Journal:  J Biol Chem       Date:  1961-04       Impact factor: 5.157

2.  Spectral-absorption coefficients of some porphyrins in the Soret-band region.

Authors:  C Rimington
Journal:  Biochem J       Date:  1960-06       Impact factor: 3.857

3.  A new precise and sensitive method for the assay of urine porphyrins using combined thin-layer chromatography and spectrophotofluorimetric analysis.

Authors:  A Pinelli; R Gaspari
Journal:  Clin Chim Acta       Date:  1972-06       Impact factor: 3.786

4.  Isolation of porphyrins from porphyria urine by preparative thin-layer chromatography.

Authors:  R A Cardinal; I Bossenmaier; Z J Petryka; L Johnson; C J Watson
Journal:  J Chromatogr       Date:  1968-11-05

5.  Thin-layer chromatography of free and esterified porphyrins on talc.

Authors:  T K With
Journal:  J Chromatogr       Date:  1969-07

6.  [A simple method of determining delta-amino-levulino-dehydratase in the blood. Behavior of the enzyme in lead poisoning].

Authors:  D Bonsignore; P Calissano; C Cartasegna
Journal:  Med Lav       Date:  1965-03       Impact factor: 1.275

  6 in total

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