Literature DB >> 6298717

A small DNA molecule containing covalently-linked ribonucleotides originates from the large intergenic region of the cauliflower mosaic virus genome.

S N Covey, D Turner, G Mulder.   

Abstract

We have detected a small DNA molecule (sa-DNA), 725 nucleotides long, in cauliflower mosaic virus (CaMV)-infected, but not non-infected, turnip leaves. Alkali and RNase A treatments shortened sa-DNA by 100 nucleotides and we conclude that it contains covalently-linked ribonucleotides. This DNA co-purified with cellular polyadenylated RNA. It is complementary to the beta-strand of CaMV DNA and of opposite polarity to RNAs transcribed from the alpha-strand. Hybridisation studies suggest that sa-DNA originates from the large intergenic region (IR1) of the CaMV genome. A small double-stranded DNA with three single-stranded components, which co-purifies with cellular DNA, appears to be related to sa-DNA but lacks detectable ribonucleotides.

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Year:  1983        PMID： 6298717      PMCID： PMC325712          DOI： 10.1093/nar/11.2.251

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

3.  Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.

Authors:  J Favaloro; R Treisman; R Kamen
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

4.  Structure of polyoma virus late nuclear RNA.

Authors:  R Treisman; R Kamen
Journal:  J Mol Biol       Date:  1981-05-25       Impact factor: 5.469

5.  Nucleotide sequence of cauliflower mosaic virus DNA.

Authors:  A Franck; H Guilley; G Jonard; K Richards; L Hirth
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

6.  Replication of cauliflower mosaic virus and transcription of its genome in turnip leaf protoplasts.

Authors:  S H Howell; R Hull
Journal:  Virology       Date:  1978-05-15       Impact factor: 3.616

7.  Subcellular distribution and properties of poly(A)-containing RNA from cultured plant cells.

Authors:  S N Covey; D Grierson
Journal:  Eur J Biochem       Date:  1976-04-01

8.  Studies on the single-stranded discontinuities of the cauliflower mosaic virus genome.

Authors:  M Volovitch; C Drugeon; P Yot
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

9.  The polarity of the cauliflower mosaic virus genome.

Authors:  R Hull; S N Covey; J Stanley; J W Davies
Journal:  Nucleic Acids Res       Date:  1979-10-10       Impact factor: 16.971

10.  The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing.

Authors:  R C Gardner; A J Howarth; P Hahn; M Brown-Luedi; R J Shepherd; J Messing
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971
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  13 in total

1.  Nucleic acids in mummified plant seeds: screening of twelve specimens by gel-electrophoresis, molecular hybridization and DNA cloning.

Authors:  F Rollo; A La Marca; A Amici
Journal:  Theor Appl Genet       Date:  1987-02       Impact factor: 5.699

2.  Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (-) strand viral DNA and reverse transcription of primed RNA templates.

Authors:  C M Thomas; R Hull; J A Bryant; A J Maule
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

3.  An analysis of the sequence of an infectious clone of rice tungro bacilliform virus, a plant pararetrovirus.

Authors:  J M Hay; M C Jones; M L Blakebrough; I Dasgupta; J W Davies; R Hull
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

4.  Nuclei purified from cauliflower mosaic virus-infected turnip leaves contain subgenomic, covalently closed circular cauliflower mosaic virus DNAs.

Authors:  N E Olszewski; T J Guilfoyle
Journal:  Nucleic Acids Res       Date:  1983-12-20       Impact factor: 16.971

5.  Identification and characterization of the woodchuck hepatitis virus origin of DNA replication.

Authors:  C Seeger; J Maragos
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

6.  Evidence from cauliflower mosaic virus virion DNA for additional discontinuities in the plus strand.

Authors:  A J Maule; C M Thomas
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

7.  Intracellular forms of viral DNA consistent with a model of reverse transcriptional replication of the cauliflower mosaic virus genome.

Authors:  Y Marco; S H Howell
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

8.  Host regulation of the cauliflower mosaic virus multiplication cycle.

Authors:  S N Covey; D S Turner; A P Lucy; K Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

9.  Partial characterisation of different classes of viral DNA, and kinetics of DNA synthesis in turnip protoplasts infected with cauliflower mosaic virus.

Authors:  A J Maule
Journal:  Plant Mol Biol       Date:  1985-01       Impact factor: 4.076

10.  Characterisation of cauliflower mosaic virus DNA forms isolated from infected turnip leaves.

Authors:  R Hull; S N Covey
Journal:  Nucleic Acids Res       Date:  1983-03-25       Impact factor: 16.971
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