Literature DB >> 629767

A theoretical analysis of the use of zonal gel filtration in the detection and purification of protein-ligand complexes.

I A Nimmo, A Bauermeister.   

Abstract

A simple model of zonal gel filtration is analysed numerically to establish semiquantitatively how the capacity of a protein-ligand complex to survive gel filtration intact depends on several experimental variables and on the kinetic characteristics of the complex. The design of gel-filtration experiments aimed at detecting and partially purifying binding proteins is discussed.

Mesh：

Substances：

Year:  1978        PMID： 629767      PMCID： PMC1184186          DOI： 10.1042/bj1690437

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  Ligandin.

Authors:  B Ketterer; E Tipping; J Meuwissen; D Beale
Journal:  Biochem Soc Trans       Date:  1975       Impact factor: 5.407

2.  Active enzyme gel chromatography: II. Computer simulations.

Authors:  B B Brown; J K Zimmerman
Journal:  Biophys Chem       Date:  1976-09       Impact factor: 2.352

Review 3.  Mechanism of action of the female sex hormones.

Authors:  E V Jensen; E R DeSombre
Journal:  Annu Rev Biochem       Date:  1972       Impact factor: 23.643

4.  Glucocorticoid receptors: relations between steroid binding and biological effects.

Authors:  G G Rousseau; J D Baxter; G M Tomkins
Journal:  J Mol Biol       Date:  1972-06-14       Impact factor: 5.469

5.  An experimental and theoreticl investigation of boundary spreading in gel chromatography.

Authors:  G E Hibberd; A G Ogston; D J Winzor
Journal:  J Chromatogr       Date:  1970-05-06

Review 6.  The application of gel filtration to the study of protein-binding of small molecules.

Authors:  G C Wood; P F Cooper
Journal:  Chromatogr Rev       Date:  1970-01

7.  Purification of an estrophilic protein from calf uterus.

Authors:  E R De Sombre; G A Puca; E V Jensen
Journal:  Proc Natl Acad Sci U S A       Date:  1969-09       Impact factor: 11.205

8.  Binding of bile acids by 100 000g supernatants from rat liver.

Authors:  R C Strange; I A Nimmo; I W Percy-Robb
Journal:  Biochem J       Date:  1977-03-15       Impact factor: 3.857

9.  Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions.

Authors:  A J Levi; Z Gatmaitan; I M Arias
Journal:  J Clin Invest       Date:  1969-11       Impact factor: 14.808

10.  Fatty acid-binding protein in small intestine. Identification, isolation, and evidence for its role in cellular fatty acid transport.

Authors:  R K Ockner; J A Manning
Journal:  J Clin Invest       Date:  1974-08       Impact factor: 14.808
View more
  4 in total

1.  Re-evaluation of the glycerol-3-phosphate dehydrogenase/L-lactate dehydrogenase enzyme system. Evidence against the direct transfer of NADH between active sites.

Authors:  S P Brooks; K B Storey
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

2.  rRNA topography in ribosome. IV. The accessibility of the 5'-end region of 16S rRNA.

Authors:  E A Skripkin; A M Kopylov; A A Bogdanov; S V Vinogradov; Y A Berlin
Journal:  Mol Biol Rep       Date:  1979-12-31       Impact factor: 2.316

3.  Analysis of protein-protein interaction by simulation of small-zone size exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed high-performance liquid chromatography elution characteristics.

Authors:  F J Stevens
Journal:  Biophys J       Date:  1989-06       Impact factor: 4.033

4.  A Cytokinin-binding Protein from Wheat Germ: Isolation by Affinity Chromatography and Properties.

Authors:  F H Moore
Journal:  Plant Physiol       Date:  1979-10       Impact factor: 8.340
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.