Literature DB >> 629750

Anomalous migration of leghaemoglobin on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

P Lehtovaara.   

Abstract

The estimate of the molecular weight of leghaemoglobin by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis is about 20% too low. This is due to an anomalously high limiting relative mobility. Leghaemoglobin binds 1.4 g of sodium dodecyl sulphate/g of protein with a concomitant decrease in the helical content from 71-72% to 49-51%.

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Year:  1978        PMID： 629750      PMCID： PMC1184216          DOI： 10.1042/bj1690251

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Studies on ligand binding of kidney bean leghemoglobin.

Authors:  P Lehtovaara
Journal:  Acta Chem Scand B       Date:  1977

2.  Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.

Authors:  G A Banker; C W Cotman
Journal:  J Biol Chem       Date:  1972-09-25       Impact factor: 5.157

3.  Relative importance of some factors affecting the electrophoretic migration of proteins in sodium dodecyl sulfate-polyacrylamide gels.

Authors:  J S Tung; C A Knight
Journal:  Anal Biochem       Date:  1972-07       Impact factor: 3.365

4.  Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.

Authors:  Y H Chen; J T Yang; H M Martinez
Journal:  Biochemistry       Date:  1972-10-24       Impact factor: 3.162

5.  Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.

Authors:  J P Segrest; R L Jackson; E P Andrews; V T Marchesi
Journal:  Biochem Biophys Res Commun       Date:  1971-07-16       Impact factor: 3.575

6.  Characterization of collagen peptides by sodium dodecylsulfate-polyacrylamide electrophoresis.

Authors:  H Furthmayr; R Timpl
Journal:  Anal Biochem       Date:  1971-06       Impact factor: 3.365

7.  Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.

Authors:  R T Swank; K D Munkres
Journal:  Anal Biochem       Date:  1971-02       Impact factor: 3.365

8.  The binding of sodium dodecyl sulphate to various proteins.

Authors:  R Pitt-Rivers; F S Impiombato
Journal:  Biochem J       Date:  1968-10       Impact factor: 3.857

9.  Binding isotherms of sodium dodecyl sulfate to protein polypeptides with special reference to SDS-polyacylamide gel electrophoresis.

Authors:  T Takagi; K Tsujii; K Shirahama
Journal:  J Biochem       Date:  1975-05       Impact factor: 3.387

10.  Free-boundary electrophoresis of sodium dodecyl sulfate-protein polypeptide complexes with special reference to SDS-polyacrylamide gel electrophoresis.

Authors:  K Shirahama; K Tsujii; T Takagi
Journal:  J Biochem       Date:  1974-02       Impact factor: 3.387
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  3 in total

1.  Protein Carbonylation and Glycation in Legume Nodules.

Authors:  Manuel A Matamoros; Ahyoung Kim; María Peñuelas; Christian Ihling; Eva Griesser; Ralf Hoffmann; Maria Fedorova; Andrej Frolov; Manuel Becana
Journal:  Plant Physiol       Date:  2018-07-03       Impact factor: 8.340

2.  Purificaton of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and resolution of its four component polypeptides.

Authors:  C E Henderson; R N Perham
Journal:  Biochem J       Date:  1980-07-01       Impact factor: 3.857

3.  Human eosinophil arylsulfatase B. Structure and activity of the purified tetrameric lysosomal hydrolase.

Authors:  P F Weller; K F Austen
Journal:  J Clin Invest       Date:  1983-01       Impact factor: 14.808
  3 in total

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