Literature DB >> 6297457

The inhibition of bovine and rat parotid deoxyribonuclease I by skeletal muscle actin. A biochemical and immunocytochemical study.

H G Mannherz, V Kreuder, J Koch, J Dieckhoff, D Drenckhahn.   

Abstract

Rat and bovine parotid gland and pancreas contain deoxyribonuclease I (DNAase I) activities in different amounts. The DNAase I activity in tissue homogenates of bovine and rat parotid gland can be inhibited by addition of monomeric actin, as with the enzyme of bovine pancreas. The isolated DNAase I species from bovine and rat parotid gland differ in their molecular weights and also in their affinities for monomeric actin, being lowest for rat parotid DNAase I (5 X 10(6)M(-1). Antibodies raised against rat and bovine parotid and bovine pancreatic DNAase I can be used to study the subcellular localization of DNAase I in these tissues by indirect immunofluorescence. DNAase I was found to be confined solely to the secretory granules of the tissue from which it was isolated.

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Year:  1982        PMID： 6297457      PMCID： PMC1153861          DOI： 10.1042/bj2070305

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  Nuclease detection in SDS-polyacrylamide gel electrophoresis.

Authors:  A L Rosenthal; S A Lacks
Journal:  Anal Biochem       Date:  1977-05-15       Impact factor: 3.365

2.  A specific 1:1 G-actin:DNAase i complex formed by the action of DNAase I on F-actin.

Authors:  H G Mannherz; J B Leigh; R Leberman; H Pfrang
Journal:  FEBS Lett       Date:  1975-12-01       Impact factor: 4.124

3.  Purification and properties of 5'-nucleotidase from lymphocyte plasma membranes.

Authors:  J Dornand; J C Bonnafous; J C Mani
Journal:  Eur J Biochem       Date:  1978-07-03

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Preparation and properties of 5'-nucleotidase from smooth muscle of small intestine.

Authors:  R M Burger; J M Lowenstein
Journal:  J Biol Chem       Date:  1970-12-10       Impact factor: 5.157

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Isolation and characterization of secretory actin . DNAase I complex from rat pancreatic juice.

Authors:  G Rohr; H G Mannherz
Journal:  Eur J Biochem       Date:  1978-08-15

8.  5'-Nucleotidase reverses the inhibitory action of actin on pancreatic deoxyribonuclease I.

Authors:  H G Mannherz; G Rohr
Journal:  FEBS Lett       Date:  1978-11-15       Impact factor: 4.124

9.  Purification and properties of human pancreatic deoxyribonuclease I.

Authors:  A Funakoshi; Y Tsubota; H Wakasugi; H Ibayashi; Y Takagi
Journal:  J Biochem       Date:  1977-12       Impact factor: 3.387

10.  Immunocytochemical localization of secretory proteins in bovine pancreatic exocrine cells.

Authors:  J P Kraehenbuhl; L Racine; J D Jamieson
Journal:  J Cell Biol       Date:  1977-02       Impact factor: 10.539
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  4 in total

1.  Engineering actin-resistant human DNase I for treatment of cystic fibrosis.

Authors:  J S Ulmer; A Herzka; K J Toy; D L Baker; A H Dodge; D Sinicropi; S Shak; R A Lazarus
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

2.  Localization of deoxyribonuclease I gene transcripts and protein in rat tissues and its correlation with apoptotic cell elimination.

Authors:  S Zanotti; B Polzar; H Stephan; U Doll; J Niessing; H G Mannherz
Journal:  Histochem Cell Biol       Date:  1995-05       Impact factor: 4.304

3.  Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse.

Authors:  Markus Napirei; Albert Ricken; Dirk Eulitz; Heiko Knoop; Hans Georg Mannherz
Journal:  Biochem J       Date:  2004-06-15       Impact factor: 3.857

4.  Androgen ablation leads to an upregulation and intranuclear accumulation of deoxyribonuclease I in rat prostate epithelial cells paralleling their apoptotic elimination.

Authors:  F Rauch; B Polzar; H Stephan; S Zanotti; R Paddenberg; H G Mannherz
Journal:  J Cell Biol       Date:  1997-05-19       Impact factor: 10.539
  4 in total

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