Literature DB >> 6296041

In vivo packaging of cosmids in transposon-mediated mutagenesis.

F F White, H J Klee, E W Nester.   

Abstract

A technique was developed that permits the analysis of large regions of DNA by transposition mutagenesis. Large fragments of the pTiA6NC plasmid were cloned into the broad host range cosmid pHK17 and subjected to transposition mutagenesis by Tn3. Cosmids containing Tn3 insertions were selected by in vivo packaging by lambda cI857 and transduction to a new host. The insertions were localized by DNA restriction endonuclease analysis and transferred to the Ti-plasmid by marker exchange.

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Year:  1983        PMID： 6296041      PMCID： PMC221737          DOI： 10.1128/jb.153.2.1075-1078.1983

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.

Authors:  M D Chilton; M H Drummond; D J Merio; D Sciaky; A L Montoya; M P Gordon; E W Nester
Journal:  Cell       Date:  1977-06       Impact factor: 41.582

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens.

Authors:  G Ooms; P M Klapwijk; J A Poulis; R A Schilperoort
Journal:  J Bacteriol       Date:  1980-10       Impact factor: 3.490

5.  Packaging of plasmid DNA containing the cohesive ends of coliphage lambda.

Authors:  H J Vollenweider; M Fiandt; E C Rosenvold; W Szybalski
Journal:  Gene       Date:  1980-04       Impact factor: 3.688

6.  Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors:  J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal:  J Bacteriol       Date:  1976-09       Impact factor: 3.490

7.  Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.

Authors:  D J Garfinkel; E W Nester
Journal:  J Bacteriol       Date:  1980-11       Impact factor: 3.490

8.  A general method for site-directed mutagenesis in prokaryotes.

Authors:  G B Ruvkun; F M Ausubel
Journal:  Nature       Date:  1981-01-01       Impact factor: 49.962

9.  Integration and organization of Ti plasmid sequences in crown gall tumors.

Authors:  M F Thomashow; R Nutter; A L Montoya; M P Gordon; E W Nester
Journal:  Cell       Date:  1980-03       Impact factor: 41.582

10.  DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.

Authors:  F Heffron; B J McCarthy; H Ohtsubo; E Ohtsubo
Journal:  Cell       Date:  1979-12       Impact factor: 41.582
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  10 in total

1.  Cosmid-based system for transient expression and absolute off-to-on transcriptional control of Escherichia coli genes.

Authors:  John E Cronan
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

2.  Cloning and sequence analysis of a trans-regulatory locus required for exoenzyme S synthesis in Pseudomonas aeruginosa.

Authors:  D W Frank; B H Iglewski
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

3.  In vivo repackaging of recombinant cosmid molecules for analyses of Salmonella typhimurium, Streptococcus mutans, and mycobacterial genomic libraries.

Authors:  W R Jacobs; J F Barrett; J E Clark-Curtiss; R Curtiss
Journal:  Infect Immun       Date:  1986-04       Impact factor: 3.441

4.  Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid.

Authors:  H J Klee; F F White; V N Iyer; M P Gordon; E W Nester
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

5.  Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region.

Authors:  C J Douglas; R J Staneloni; R A Rubin; E W Nester
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

6.  Method for gene replacement in Pseudomonas aeruginosa used in construction of recA mutants: recA-independent instability of alginate production.

Authors:  D E Ohman; M A West; J L Flynn; J B Goldberg
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

7.  Cloning of the recA gene of Neisseria gonorrhoeae and construction of gonococcal recA mutants.

Authors:  J M Koomey; S Falkow
Journal:  J Bacteriol       Date:  1987-02       Impact factor: 3.490

8.  Cloning and sequencing of the Pseudomonas aeruginosa 1244 pilin structural gene.

Authors:  P A Castric; H F Sidberry; J C Sadoff
Journal:  Mol Gen Genet       Date:  1989-03

9.  Bacteriophage SPO2-mediated plasmid transduction in transpositional mutagenesis within the genus Bacillus.

Authors:  L K Kopec; R E Yasbin; R Marrero
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

10.  A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.

Authors:  S E Stachel; G An; C Flores; E W Nester
Journal:  EMBO J       Date:  1985-04       Impact factor: 11.598
  10 in total

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