Literature DB >> 6294478

Marker rescue allows direct selection for recombinant plasmids in streptococci.

D Behnke.   

Abstract

Resident deletion derivatives (Ems or Cms) of the streptococcal plasmid vector pGB301 rescue antibiotic resistance genes from linearized pGB301 (Emr, Cmr) DNA with high frequency. Insertion of passenger DNA next to an antibiotic resistance determinant of pGB301, which is missing on the resident plasmid, forces corescue of these two plasmid domains, thus allowing direct selection for recombinant plasmids.

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Substances：

Year:  1982        PMID： 6294478     DOI： 10.1007/bf00333012

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  10 in total

1.  Marker rescue transformation by linear plasmid DNA in Bacillus subtilis.

Authors:  S Contente; D Dubnau
Journal:  Plasmid       Date:  1979-10       Impact factor: 3.466

2.  Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid in Bacillus subtilis.

Authors:  T Gryczan; S Contente; D Dubnau
Journal:  Mol Gen Genet       Date:  1980-02

3.  Location of antibiotic resistance determinants, copy control, and replication functions on the double-selective streptococcal cloning vector pGB301.

Authors:  D Behnke; M S Gilmore
Journal:  Mol Gen Genet       Date:  1981

4.  Molecular cloning in streptococci: physical mapping of the vehicle plasmid pSM10 and demonstration of intergroup DNA transfer.

Authors:  H Malke; L G Burman; S E Holm
Journal:  Mol Gen Genet       Date:  1981

5.  Pathway of plasmid transformation in Pneumococcus: open circular and linear molecules are active.

Authors:  C W Saunders; W R Guild
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

6.  Molecular cloning of an erythromycin resistance determinant in streptococci.

Authors:  D Behnke; J J Ferretti
Journal:  J Bacteriol       Date:  1980-11       Impact factor: 3.490

7.  Plasmid transformation of Streptococcus sanguis (Challis) occurs by circular and linear molecules.

Authors:  D Behnke
Journal:  Mol Gen Genet       Date:  1981

8.  Transformation of Streptococcus sanguis with monomeric pVA736 plasmid deoxyribonucleic acid.

Authors:  F L Macrina; K R Jones; R A Welch
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

9.  Chimeric streptococcal plasmids and their use as molecular cloning vehicles in Streptococcus sanguis (Challis).

Authors:  F L Macrina; K R Jones; P H Wood
Journal:  J Bacteriol       Date:  1980-09       Impact factor: 3.490

10.  Plasmid pGB301, a new multiple resistance streptococcal cloning vehicle and its use in cloning of a gentamicin/kanamycin resistance determinant.

Authors:  D Behnke; M S Gilmore; J J Ferretti
Journal:  Mol Gen Genet       Date:  1981
  10 in total
  1 in total

1.  Establishment of an improved high-efficiency thermal asymmetric interlaced PCR for identification of genomic integration sites mediated by phiC31 integrase.

Authors:  Zaiwei Zhou; Haiyan Ma; Lijuan Qu; Fei Xie; Qingwen Ma; Zhaorui Ren
Journal:  World J Microbiol Biotechnol       Date:  2011-09-10       Impact factor: 3.312
  1 in total

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