Literature DB >> 6294455

Two-protonic-state electrophiles as probes of enzyme mechanisms.

K Brocklehurst.   

Abstract

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Year:  1982        PMID： 6294455     DOI： 10.1016/s0076-6879(82)87026-2

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


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  24 in total

1.  Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine.

Authors:  T Krell; J Maclean; D J Boam; A Cooper; M Resmini; K Brocklehurst; S M Kelly; N C Price; A J Lapthorn; J R Coggins
Journal:  Protein Sci       Date:  2001-06       Impact factor: 6.725

2.  Inactivation of skeletal-muscle UDP-glucose pyrophosphorylase by reaction with carboxylate-directed reagents.

Authors:  M Signorini; C Ferrari; E Mariotti; F Dallocchio; C M Bergamini
Journal:  Biochem J       Date:  1989-12-15       Impact factor: 3.857

3.  Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.

Authors:  E Salih; J P Malthouse; D Kowlessur; M Jarvis; M O'Driscoll; K Brocklehurst
Journal:  Biochem J       Date:  1987-10-01       Impact factor: 3.857

4.  Temperature-dependences of the kinetics of reactions of papain and actinidin with a series of reactivity probes differing in key molecular recognition features.

Authors:  Sheraz Gul; Geoffrey W Mellor; Emrys W Thomas; Keith Brocklehurst
Journal:  Biochem J       Date:  2006-05-15       Impact factor: 3.857

5.  Variation in the P2-S2 stereochemical selectivity towards the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides among the cysteine proteinases papain, ficin and actinidin.

Authors:  M Patel; I S Kayani; G W Mellor; S Sreedharan; W Templeton; E W Thomas; M Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1992-01-15       Impact factor: 3.857

6.  Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives.

Authors:  B S Baines; K Brocklehurst; P R Carey; M Jarvis; E Salih; A C Storer
Journal:  Biochem J       Date:  1986-01-01       Impact factor: 3.857

7.  Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega.

Authors:  K Brocklehurst; E Salih; R McKee; H Smith
Journal:  Biochem J       Date:  1985-06-01       Impact factor: 3.857

8.  Natural structural variation in enzymes as a tool in the study of mechanism exemplified by a comparison of the catalytic-site structure and characteristics of cathepsin B and papain. pH-dependent kinetics of the reactions of cathepsin B from bovine spleen and from rat liver with a thiol-specific two-protonic-state probe (2,2'-dipyridyl disulphide) and with a specific synthetic substrate (N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine 2-naphthylamide).

Authors:  F Willenbrock; K Brocklehurst
Journal:  Biochem J       Date:  1984-09-15       Impact factor: 3.857

9.  Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe.

Authors:  G W Mellor; E W Thomas; C M Topham; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

10.  Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.

Authors:  G W Mellor; S K Sreedharan; D Kowlessur; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857
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