Synthesis of human beta-endorphinyl-thiolglycine and its use for the preparation of affinity columns of beta-endorphin.

The peptide human beta-endorphinyl-thiolglycine (I) has been synthesized by the solid-phase method. The citraconyl derivative of peptide I was coupled to aminohexyl-Sepharose by reaction with silver nitrate/N-hydroxysuccinimide in water. The citraconyl groups were removed in aqueous acetic acid and the resulting resin was used in affinity chromatography for the purification of antisera to beta-lipotropin and beta-endorphin.