Literature DB >> 6293413

Serotyping and subgrouping of rotavirus strains by the ELISA test.

M E Thouless, G M Beards, T H Flewett.   

Abstract

A method is described for the serotyping and subgrouping of rotaviruses by enzyme-linked immuno-sorbent assay (ELISA). For the isolates tested "blind" under code in parallel the serotyping results obtained by ELISA and serum neutralization of fluorescent focus formation were the same. After absorption of the typing antisera with purified heterotypic rotavirus, up to 128-fold differences in titres between isolates were observed in the ELISA test. The results of serotyping and subgrouping, by ELISA, of strains previously described and characterized by the authors' and other laboratories are also given. An attempt is made to correlate the serological findings with variations between virus strains based upon patterns of genome molecular weights as revealed by polyacrylamide gel electrophoresis. The application of this ELISA technique to epidemiological studies and vaccine research is discussed.

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Year:  1982        PMID： 6293413     DOI： 10.1007/bf01318076

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  21 in total

1.  Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens.

Authors:  A Z Kapikian; W L Cline; H B Greenberg; R G Wyatt; A R Kalica; C E Banks; H D James; J Flores; R M Chanock
Journal:  Infect Immun       Date:  1981-08       Impact factor: 3.441

2.  Location of type-specific antigens in calf rotaviruses.

Authors:  J C Bridger
Journal:  J Clin Microbiol       Date:  1978-12       Impact factor: 5.948

3.  More serotypes of human rotavirus.

Authors:  T H Flewett; M E Thouless; J N Pilfold; A S Bryden; J A Candeias
Journal:  Lancet       Date:  1978-09-16       Impact factor: 79.321

4.  Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2.

Authors:  A R Kalica; H B Greenberg; R T Espejo; J Flores; R G Wyatt; A Z Kapikian; R M Chanock
Journal:  Infect Immun       Date:  1981-09       Impact factor: 3.441

5.  Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences.

Authors:  R T Espejo; O Muñóz; F Serafin; P Romero
Journal:  Infect Immun       Date:  1980-02       Impact factor: 3.441

6.  Enzyme-linked immunosorbent assays adapted for serotyping of human rotavirus strains.

Authors:  G Zissis; J P Lambert
Journal:  J Clin Microbiol       Date:  1980-01       Impact factor: 5.948

7.  Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals.

Authors:  G N Woode; J C Bridger; J M Jones; T H Flewett; H A Davies; H A Davis; G B White
Journal:  Infect Immun       Date:  1976-09       Impact factor: 3.441

8.  Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid.

Authors:  S M Rodger; R F Bishop; C Birch; B McLean; I H Holmes
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948

9.  Sequential passages of human rotavirus in MA-104 cells.

Authors:  T Urasawa; S Urasawa; K Taniguchi
Journal:  Microbiol Immunol       Date:  1981       Impact factor: 1.955

10.  The rotaviruses.

Authors:  T H Flewett; G N Woode
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574
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  53 in total

1.  Expression of the major capsid protein VP6 of group C rotavirus and synthesis of chimeric single-shelled particles by using recombinant baculoviruses.

Authors:  G Tosser; M Labbé; M Brémont; J Cohen
Journal:  J Virol       Date:  1992-10       Impact factor: 5.103

2.  Detection of human rotavirus by nucleic acid analysis in comparison to enzyme-linked immunoassay and electron microscopy.

Authors:  B Selb; H G Baumeister; G Maass; H W Doerr
Journal:  Eur J Clin Microbiol       Date:  1985-02       Impact factor: 3.267

3.  Non-neutralizing monoclonal antibodies to a trypsin-sensitive site on the major glycoprotein of rotavirus which discriminate between virus serotypes.

Authors:  B S Coulson; K J Fowler; J R White; R G Cotton
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

4.  Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.

Authors:  B S Coulson; L E Unicomb; G A Pitson; R F Bishop
Journal:  J Clin Microbiol       Date:  1987-03       Impact factor: 5.948

5.  Development of a monoclonal antibody specific for serotype 3 rotavirus strains.

Authors:  B Grunert; H J Streckert; W Liedtke; C Houly; C Mietens; H Werchau
Journal:  Eur J Clin Microbiol       Date:  1987-04       Impact factor: 3.267

6.  Comparative sensitivities of solid-phase immune electron microscopy and enzyme-linked immunosorbent assay for serotyping of human rotavirus strains with neutralizing monoclonal antibodies.

Authors:  G Gerna; A Sarasini; B S Coulson; M Parea; M Torsellini; E Arbustini; M Battaglia
Journal:  J Clin Microbiol       Date:  1988-07       Impact factor: 5.948

7.  Rapid serotyping of human rotavirus strains by solid-phase immune electron microscopy.

Authors:  G Gerna; N Passarani; M Battaglía; E Percivalle
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

8.  Molecular epidemiology of human rotaviruses. Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83.

Authors:  E A Follett; R C Sanders; G M Beards; F Hundley; U Desselberger
Journal:  J Hyg (Lond)       Date:  1984-04

9.  Epidemiology of symptomatic human rotaviruses in Bangalore and Mysore, India, from 1988 to 1994 as determined by electropherotype, subgroup and serotype analysis.

Authors:  S Aijaz; K Gowda; H V Jagannath; R R Reddy; P P Maiya; R L Ward; H B Greenberg; M Raju; A Babu; C D Rao
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

10.  Subgrouping of human rotavirus strains by complement fixation, indirect double-antibody sandwich enzyme-linked immunosorbent assay and solid-phase immune electron microscopy.

Authors:  G Gerna; M Torsellini; N Passarani; M Battaglia; E Percivalle; A Sarasini; D Torre; P Ferrante
Journal:  Arch Virol       Date:  1984       Impact factor: 2.574
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