Literature DB >> 6291966

Phosphorylation changes induced by cAMP derivatives in the CHO cell and selected mutants.

E G Gabrielson, C Scoggin, T T Puck.   

Abstract

Phosphorylation analysis of CHO mutants selected for unresponsiveness to the Reverse Transformation reaction of cAMP derivatives has been carried out by 2D gel electrophoresis in hopes of finding mutants differing from the wild-type cell in a minimal number of such phosphorylations. Seven differences in protein phosphorylations in the parental CHO cell have been identified as a result of treatment with db-cAMP. db-cAMP-unresponsive mutants of two kinds have been found: One type has lost all seven of the phosphorylation changes induced by db-cAMP in the wild-type cell. The other type which is equally resistant to the Reverse Transformation response differs from the parental cell in only one (or possibly two) phosphorylation(s) involving a 55 000 D protein. This protein may, therefore, be directly related to the events of Reverse Transformation.

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Year:  1982        PMID： 6291966     DOI： 10.1016/0014-4827(82)90409-8

Source DB:  PubMed          Journal:  Exp Cell Res        ISSN： 0014-4827            Impact factor:   3.905


  6 in total

1.  The spatial distribution of exposed nuclear DNA in normal, cancer, and reverse-transformed cells.

Authors:  A Krystosek; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

2.  Involvement of vimentin in the reverse transformation reaction.

Authors:  D Chan; A Goate; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

3.  Localization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11.

Authors:  P Cheung; F T Kao; M L Law; C Jones; T T Puck; L Chan
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

4.  Cyclic AMP and theophylline enhance DNA synthesis in L cells stimulated with anti-actin IgG and [(IgG)2 protein A]2 complex by recruiting cells into S-phase.

Authors:  W T Shearer; R G Ulrich; J E McClure; C G Green; E B Gilliam; C Das; J J Langone
Journal:  Mol Cell Biochem       Date:  1985-07       Impact factor: 3.396

5.  Dibutyryl cAMP-inducible alkaline phosphatase in animal cell plasma membranes: fluorescence detection of mutant clones on polyester cloth.

Authors:  J R Gum; C R Raetz
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

6.  Modulation of vimentin containing intermediate filament distribution and phosphorylation in living fibroblasts by the cAMP-dependent protein kinase.

Authors:  N J Lamb; A Fernandez; J R Feramisco; W J Welch
Journal:  J Cell Biol       Date:  1989-06       Impact factor: 10.539
  6 in total

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