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Literature DB >> 6291941

The intracellular concentration of pyrophosphate in the batch culture of Escherichia coli.

Abstract

We applied our colorimetric PPi determination method [Heinonen, J., Honkasalo, S. and Kukko, E. (1981) Anal. Biochem. 117, 293-300] to bacterial cultures and measured the intracellular concentration of PPi in the batch culture of Escherichia coli. The cells growing in minimal medium contained about 2.5 nmol PPi/mg protein (0.5 mM). There was no extracellular PPi. A similar concentration of PPi was found also in cells growing in minimal medium enriched by amino acid mixture.

Entities: Chemical Species

Mesh：

Substances：
Diphosphates

Year: 1982 PMID： 6291941 DOI： 10.1111/j.1432-1033.1982.tb06878.x

Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956

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Cited

16 in total

1. Pyrophosphatase is essential for growth of Escherichia coli.

Authors: J Chen; A Brevet; M Fromant; F Lévêque; J M Schmitter; S Blanquet; P Plateau
Journal: J Bacteriol Date: 1990-10 Impact factor: 3.490

2. Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.

Authors: E Kukko-Kalske; M Lintunen; M K Inen; R Lahti; J Heinonen
Journal: J Bacteriol Date: 1989-08 Impact factor: 3.490

3. Thermodynamic favorability and pathway yield as evolutionary tradeoffs in biosynthetic pathway choice.

Authors: Bin Du; Daniel C Zielinski; Jonathan M Monk; Bernhard O Palsson
Journal: Proc Natl Acad Sci U S A Date: 2018-10-11 Impact factor: 11.205

4. The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.

Authors: Masahiko Imashimizu; Maria L Kireeva; Lucyna Lubkowska; Mikhail Kashlev; Nobuo Shimamoto
Journal: J Mol Biol Date: 2019-04-26 Impact factor: 5.469

5. Cellular Concentrations of Nucleotide Diphosphate-Chelated Magnesium Ions Accelerate Catalysis by RNA and DNA Enzymes.

Authors: Ryota Yamagami; Ruochuan Huang; Philip C Bevilacqua
Journal: Biochemistry Date: 2019-09-12 Impact factor: 3.162

6. Copper nanoclusters capped with tannic acid as a fluorescent probe for real-time determination of the activity of pyrophosphatase.

Authors: Qing Liu; Qi Lai; Ning Li; Xingguang Su
Journal: Mikrochim Acta Date: 2018-02-17 Impact factor: 5.833

The intracellular concentration of pyrophosphate in the batch culture of Escherichia coli.

1. Pyrophosphatase is essential for growth of Escherichia coli.

2. Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.

3. Thermodynamic favorability and pathway yield as evolutionary tradeoffs in biosynthetic pathway choice.

4. The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.

5. Cellular Concentrations of Nucleotide Diphosphate-Chelated Magnesium Ions Accelerate Catalysis by RNA and DNA Enzymes.

6. Copper nanoclusters capped with tannic acid as a fluorescent probe for real-time determination of the activity of pyrophosphatase.

7. Structural and biochemical studies on the regulation of biotin carboxylase by substrate inhibition and dimerization.

8. Diphosphate concentration does not correlate with the level of inorganic diphosphatase in Escherichia coli.

9. Accumulation of pyrophosphate in Escherichia coli. Relationship to growth and nucleotide synthesis.

10. Comparative kinetic studies on the two interconvertible forms of Streptococcus faecalis inorganic pyrophosphatase.