Literature DB >> 6290466

Antibody to the nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is specific for polyoma middle T antigen and inhibits in vitro kinase activity.

B Schaffhausen, T L Benjamin, L Pike, J Casnellie, E Krebs.   

Abstract

Middle T antigen of polyoma virus has an associated tyrosine kinase activity which phosphorylates tyrosine residue 315 on middle T in immunoprecipitates. A peptide representing the sequence of middle T from residue 311 to 319 has been synthesized. This peptide acts as a weak inhibitor of the kinase reaction. An antiserum has been raised against this peptide after conjugation to bovine serum albumin. The antibody is middle T-specific. Middle T antigen precipitated by this serum is largely inactive in the kinase reaction. Dissociation of the immune complex with peptide releases middle T in a kinase-active form.

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Year:  1982        PMID： 6290466

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  14 in total

1.  Tomato Rab1A homologs as molecular tools for studying Rab geranylgeranyl transferase in plant cells.

Authors:  A E Loraine; S Yalovsky; S Fabry; W Gruissem
Journal:  Plant Physiol       Date:  1996-04       Impact factor: 8.340

2.  Polyomavirus small t antigen: overproduction in bacteria, purification, and utilization for monoclonal and polyclonal antibody production.

Authors:  D C Pallas; C Schley; M Mahoney; E Harlow; B S Schaffhausen; T M Roberts
Journal:  J Virol       Date:  1986-12       Impact factor: 5.103

3.  Proteomic analysis of scaffold proteins in the ERK cascade.

Authors:  Melissa M McKay; Deborah K Morrison
Journal:  Methods Mol Biol       Date:  2010

4.  Generation of protein-reactive antibodies by short peptides is an event of high frequency: implications for the structural basis of immune recognition.

Authors:  H L Niman; R A Houghten; L E Walker; R A Reisfeld; I A Wilson; J M Hogle; R A Lerner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

5.  Identification of the tyrosine protein kinase from LSTRA cells by use of site-specific antibodies.

Authors:  J E Casnellie; L E Gentry; L R Rohrschneider; E G Krebs
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

6.  Transformation by polyoma virus is drastically reduced by substitution of phenylalanine for tyrosine at residue 315 of middle-sized tumor antigen.

Authors:  G Carmichael; B S Schaffhausen; G Mandel; T J Liang; T L Benjamin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

7.  Antibodies against a nonapeptide of polyomavirus middle T antigen: cross-reaction with a cellular protein(s).

Authors:  Y Ito; Y Hamagishi; K Segawa; T Dalianis; E Appella; M Willingham
Journal:  J Virol       Date:  1983-12       Impact factor: 5.103

8.  Specific Prenylation of Tomato Rab Proteins by Geranylgeranyl Type-II Transferase Requires a Conserved Cysteine-Cysteine Motif.

Authors:  S. Yalovsky; A. E. Loraine; W. Gruissem
Journal:  Plant Physiol       Date:  1996-04       Impact factor: 8.340

9.  Construction and functional characterization of polyomavirus genomes that separately encode the three early proteins.

Authors:  Z Y Zhu; G M Veldman; A Cowie; A Carr; B Schaffhausen; R Kamen
Journal:  J Virol       Date:  1984-07       Impact factor: 5.103

10.  Complexes of polyoma virus medium T antigen and cellular proteins.

Authors:  T Grussenmeyer; K H Scheidtmann; M A Hutchinson; W Eckhart; G Walter
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205
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