Literature DB >> 6290461

Cloning of the trp-1 gene from neurospora crassa by complementation of a trpC mutation in Escherichia coli.

J K Keesey, J A Demoss.   

Abstract

Studies with a hybrid plasmid containing 4.0 kilobase pairs of Neurospora crassa DNA cloned into plasmid pBR322 indicated that the plasmid restored to prototrophy a trpC mutant of Escherichia coli which lacked phosphoribosyl anthranilate isomerase but not a trpC mutant which lacked indole glycerol phosphate synthetase, that the relevant transcription was initiated at a promoter within the N. crassa DNA, and that the phosphoribosyl anthranilate isomerase could be specified by a subcloned segment of the original DNA.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6290461      PMCID： PMC221561          DOI： 10.1128/jb.152.2.954-958.1982

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

1.  Simple method for identification of plasmid-coded proteins.

Authors:  A Sancar; A M Hack; W D Rupp
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

2.  Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

3.  The isolation of the components of the anthranilate synthetase complex from Neurospora crassa.

Authors:  A Arroyo-Begovich; J A DeMoss
Journal:  J Biol Chem       Date:  1973-02-25       Impact factor: 5.157

4.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

5.  The nonenzymatic preparation in solution of N-(5'-phosphoribosyl) anthranilic acid, an intermediate in tryptophan biosynthesis.

Authors:  T E Creighton
Journal:  J Biol Chem       Date:  1968-11-10       Impact factor: 5.157

6.  Subunit structure of anthranilate synthase from Neurospora crassa: preparation and characterization of a protease-free form.

Authors:  J Keesey; J Paukert; J A Demoss
Journal:  Arch Biochem Biophys       Date:  1981-03       Impact factor: 4.013

7.  Limited proteolysis of N-(5'-phosphoribosyl)anthranilate isomerase: indoleglycerol phosphate synthase from Escherichia coli yields two different enzymically active, functional domains.

Authors:  K Kirschner; H Szadkowski; A Henschen; F Lottspeich
Journal:  J Mol Biol       Date:  1980-11-15       Impact factor: 5.469

8.  Polarity and enzyme functions in mutants of the first three genes of the tryptophan operon of Escherichia coli.

Authors:  C Yanofsky; V Horn; M Bonner; S Stasiowski
Journal:  Genetics       Date:  1971-12       Impact factor: 4.562

9.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  The ovalbumin gene: alleles created by mutations in the intervening sequences of the natural gene.

Authors:  E C Lai; S L Woo; A Dugaiczyk; B W O'Malley
Journal:  Cell       Date:  1979-01       Impact factor: 41.582
View more
  3 in total

1.  Phycomyces blakesleeanus TRP1 gene: organization and functional complementation in Escherichia coli and Saccharomyces cerevisiae.

Authors:  J L Revuelta; M Jayaram
Journal:  Mol Cell Biol       Date:  1987-08       Impact factor: 4.272

Review 2.  Chromosomal loci of Neurospora crassa.

Authors:  D D Perkins; A Radford; D Newmeyer; M Björkman
Journal:  Microbiol Rev       Date:  1982-12

3.  Deletion analysis of domain independence in the TRP1 gene product of Neurospora crassa.

Authors:  M S Walker; J A DeMoss
Journal:  Mol Gen Genet       Date:  1990-08
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.