Literature DB >> 6290331

Isolation and characterization of yeast ring chromosome III by a method applicable to other circular DNAs.

R J Devenish, C S Newlon.   

Abstract

A method is described for the isolation and purification of covalently closed circular (ccc) DNA from yeast (Saccharomyces cerevisiae). Spheroplasts are lysed at pH 12.45 which denatures linear but not ccc DNA. Next, the lysate is taken through a gentle high-salt-phenol extraction to remove single-stranded DNA. The ccc DNA, recovered by ethanol precipitation, can be further studied by agarose gel electrophoresis, can be cut with restriction endonucleases and can be used to transform Escherichia coli. This method efficiently purifies large (approx. 190 kb) and small (approx. 1.5 kb, TRP1-RI Circle) circular DNAs and thus has general applicability for isolation and purification of plasmids from yeast.

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Year:  1982        PMID： 6290331     DOI： 10.1016/0378-1119(82)90166-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  31 in total

1.  Closing the gaps on human chromosome 19 revealed genes with a high density of repetitive tandemly arrayed elements.

Authors:  Sun-Hee Leem; Natalay Kouprina; Jane Grimwood; Jung-Hyun Kim; Michael Mullokandov; Young-Ho Yoon; Ji-Youn Chae; Jenna Morgan; Susan Lucas; Paul Richardson; Chris Detter; Tijana Glavina; Eddy Rubin; J Carl Barrett; Vladimir Larionov
Journal:  Genome Res       Date:  2004-01-12       Impact factor: 9.043

2.  The ARS consensus sequence is required for chromosomal origin function in Saccharomyces cerevisiae.

Authors:  A M Deshpande; C S Newlon
Journal:  Mol Cell Biol       Date:  1992-10       Impact factor: 4.272

3.  Spontaneous amplification of the ADH4 gene in Saccharomyces cerevisiae.

Authors:  M Dorsey; C Peterson; K Bray; C E Paquin
Journal:  Genetics       Date:  1992-12       Impact factor: 4.562

4.  Polymorphisms on the right arm of yeast chromosome III associated with Ty transposition and recombination events.

Authors:  J R Warmington; R P Green; C S Newlon; S G Oliver
Journal:  Nucleic Acids Res       Date:  1987-11-11       Impact factor: 16.971

5.  Analysis of a circular derivative of Saccharomyces cerevisiae chromosome III: a physical map and identification and location of ARS elements.

Authors:  C S Newlon; L R Lipchitz; I Collins; A Deshpande; R J Devenish; R P Green; H L Klein; T G Palzkill; R B Ren; S Synn
Journal:  Genetics       Date:  1991-10       Impact factor: 4.562

6.  Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR).

Authors:  M R Cancilla; J Graves; L E Matesic; R H Reeves; K M Tainton; K H Choo; M A Resnick; V L Larionov; N Y Kouprina
Journal:  Mamm Genome       Date:  1998-02       Impact factor: 2.957

7.  Mutational analysis of the consensus sequence of a replication origin from yeast chromosome III.

Authors:  J V Van Houten; C S Newlon
Journal:  Mol Cell Biol       Date:  1990-08       Impact factor: 4.272

8.  Replication and resolution of cloned poxvirus telomeres in vivo generates linear minichromosomes with intact viral hairpin termini.

Authors:  A M DeLange; M Reddy; D Scraba; C Upton; G McFadden
Journal:  J Virol       Date:  1986-08       Impact factor: 5.103

9.  The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

Authors:  C A Woolford; L B Daniels; F J Park; E W Jones; J N Van Arsdell; M A Innis
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

10.  Cloning whole bacterial genomes in yeast.

Authors:  Gwynedd A Benders; Vladimir N Noskov; Evgeniya A Denisova; Carole Lartigue; Daniel G Gibson; Nacyra Assad-Garcia; Ray-Yuan Chuang; William Carrera; Monzia Moodie; Mikkel A Algire; Quang Phan; Nina Alperovich; Sanjay Vashee; Chuck Merryman; J Craig Venter; Hamilton O Smith; John I Glass; Clyde A Hutchison
Journal:  Nucleic Acids Res       Date:  2010-03-07       Impact factor: 16.971
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