Literature DB >> 6289811

Limitations of commonly used spectrophotometric assay methods for phosphoenolypyruvate carboxykinase activity in crude extracts of muscle.

D A Duff, K Snell.   

Abstract

Phosphoenolpyruvate carboxykinase activity in crude extracts of muscle has frequently been determined by using a continuous spectrophotometric method, which is shown to grossly overestimate enzyme activity. NADH oxidation attributed to phosphoenolpyruvate carboxykinase activity in the assay is due to lactate production. Under the normal assay conditions. Na+ ions stimulate pyruvate kinase, providing pyruvate for lactate formation by lactate dehydrogenase and sufficiently to account for most of the observed NADH oxidation.

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Year:  1982        PMID： 6289811      PMCID： PMC1158560          DOI： 10.1042/bj2060147

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

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Authors:  J F KACHMAR; P D BOYER
Journal:  J Biol Chem       Date:  1953-02       Impact factor: 5.157

2.  Maximum activities and effects of fructose bisphosphate on pyruvate kinase from muscles of vertebrates and invertebrates in relation to the control of glycolysis.

Authors:  V A Zammit; I Beis; E A Newsholme
Journal:  Biochem J       Date:  1978-09-15       Impact factor: 3.857

3.  Phosphoenolpyruvate carboxykinase and pyruvate carboxylase in developing rat liver.

Authors:  F J Ballard; R W Hanson
Journal:  Biochem J       Date:  1967-09       Impact factor: 3.857

4.  Effects of temperature on ionic transport in aortas from rat and ground squirrel.

Authors:  K E Kamm; M L Zatzman; A W Jones; F E South
Journal:  Am J Physiol       Date:  1979-07

5.  Glycogen synthesis from lactate in the three types of skeletal muscle.

Authors:  J A McLane; J O Holloszy
Journal:  J Biol Chem       Date:  1979-07-25       Impact factor: 5.157

6.  Muscle phosphoenolpyruvate carboxykinase activity and alanine release in progressively starved rats.

Authors:  K Snell; D A Duff
Journal:  Int J Biochem       Date:  1979

7.  Determination of phosphoenolpyruvate carboxykinase activity with deoxyguanosine 5'-diphosphate as nucleotide substrate.

Authors:  I Petrescu; O Bojan; M Saied; O Bârzu; F Schmidt; H F Kühnle
Journal:  Anal Biochem       Date:  1979-07-15       Impact factor: 3.365

8.  Properties of pyruvate kinase and phosphoenolpyruvate carboxykinase in relation to the direction and regulation of phosphoenolpyruvate metabolism in muscles of the frog and marine invertebrates.

Authors:  V A Zammit; E A Newsholme
Journal:  Biochem J       Date:  1978-09-15       Impact factor: 3.857

9.  The enzymatic carboxylation of phosphoenolpyruvate. II. Purification and properties of liver mitochondrial phosphoenolpyruvate carboxykinase.

Authors:  H C Chang; M D Lane
Journal:  J Biol Chem       Date:  1966-05-25       Impact factor: 5.157

10.  The activities of pyruvate carboxylase, phosphoenolpyruvate carboxylase and fructose diphosphatase in muscles from vertebrates and invertebrates.

Authors:  B Crabtree; S J Higgins; E A Newsholme
Journal:  Biochem J       Date:  1972-11       Impact factor: 3.857
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  8 in total

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4.  Glyceroneogenesis is inhibited through HIV protease inhibitor-induced inflammation in human subcutaneous but not visceral adipose tissue.

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5.  Lactate metabolism in the perfused rat hindlimb.

Authors:  M Shiota; S Golden; J Katz
Journal:  Biochem J       Date:  1984-09-01       Impact factor: 3.857

6.  Branched-chain amino acid metabolism and alanine formation in rat muscles in vitro. Mitochondrial-cytosolic interrelationships.

Authors:  K Snell; D A Duff
Journal:  Biochem J       Date:  1985-02-01       Impact factor: 3.857

7.  Adrenergic inhibition of branched-chain 2-oxo acid dehydrogenase in rat diaphragm muscle in vitro.

Authors:  T N Palmer; M A Caldecourt; M C Sugden
Journal:  Biochem J       Date:  1983-10-15       Impact factor: 3.857

8.  The role of phosphoenolpyruvate carboxykinase in muscle alanine synthesis.

Authors:  T N Palmer; M A Caldecourt; J P Warner; M C Sugden
Journal:  Biochem J       Date:  1984-12-15       Impact factor: 3.857
  8 in total

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