Literature DB >> 6286237

Preparation of a monospecific antibody to purified rabbit synovial fibroblast collagenase.

C A Vater, J L Hahn, E D Harris.   

Abstract

Collagenase produced by monolayer cultures of rabbit synovial fibroblasts was purified from serum-free culture medium. Antiserum to the pure preparation was raised in sheep. The IgG fraction has been isolated and characterized and shown to be specific for collagenase. The antibody is capable of precipitating and inhibiting the activity of both latent and activated rabbit synovial fibroblast collagenase. Cross-reactivity with human antigen from rheumatoid synovial cell culture medium is described.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 6286237     DOI： 10.1016/s0174-173x(81)80034-9

Source DB:  PubMed          Journal:  Coll Relat Res        ISSN： 0174-173X


  7 in total

1.  Proteolytic activity of first trimester human placenta: localization of interstitial collagenase in villous and extravillous trophoblast.

Authors:  U M Moll; B L Lane
Journal:  Histochemistry       Date:  1990

2.  The precursor of a metalloendopeptidase from human rheumatoid synovial fibroblasts. Purification and mechanisms of activation by endopeptidases and 4-aminophenylmercuric acetate.

Authors:  Y Okada; E D Harris; H Nagase
Journal:  Biochem J       Date:  1988-09-15       Impact factor: 3.857

3.  Autoregulation of collagenase production by a protein synthesized and secreted by synovial fibroblasts: cellular mechanism for control of collagen degradation.

Authors:  C E Brinckerhoff; M C Benoit; W J Culp
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

4.  Biosynthesis and secretion of procollagenase by rabbit synovial fibroblasts. Inhibition of procollagenase secretion by monensin and evidence for glycosylation of procollagenase.

Authors:  H Nagase; C E Brinckerhoff; C A Vater; E D Harris
Journal:  Biochem J       Date:  1983-08-15       Impact factor: 3.857

5.  Proactivator-dependent activation of procollagenase induced by treatment with EGTA.

Authors:  C A Vater; H Nagase; E D Harris
Journal:  Biochem J       Date:  1986-08-01       Impact factor: 3.857

6.  Isolation of a collagenase cDNA clone and measurement of changing collagenase mRNA levels during induction in rabbit synovial fibroblasts.

Authors:  R H Gross; L A Sheldon; C F Fletcher; C E Brinckerhoff
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

7.  Purification of the neutral proteoglycan-degrading metalloproteinase from human articular cartilage tissue and its identification as stromelysin matrix metalloproteinase-3.

Authors:  Z Gunja-Smith; H Nagase; J F Woessner
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.