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Literature DB >> 6285307

Primary and secondary structure specificity of the cleavage of 'single-stranded' DNA by endonuclease Hinf I.

Abstract

The interaction of endonuclease Hinf I with single-stranded fd DNA was examined. The sizes of the cleavage products indicate that the enzyme cuts this substrate at the same sequences as double-stranded DNA (GANTC). To determine whether or not the recognition sites in single-stranded DNA have to be present in double-stranded form in order to be cleaved, DNA fragments containing complementary or non-complementary Hinf I sequences were prepared and treated as substrates. The results suggest that completely base-paired recognition sites are necessary for cleavage. Sequences surrounding the Hinf I pentanucleotides significantly modulate the reaction rates.

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Year: 1982 PMID： 6285307 PMCID： PMC320654 DOI： 10.1093/nar/10.9.2763

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

Primary and secondary structure specificity of the cleavage of 'single-stranded' DNA by endonuclease Hinf I.

1. Substrate dependence of the mechanism of EcoRI endonuclease.

2. Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.

3. Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate.

4. dna, single stranded/*metab.

5. Elimination of promoter function by base modification of DNA.

6. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.

7. Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius.

8. Cleavage site of bacteriophage fd gene II-protein in the origin of viral strand replication.

9. Nucleotide sequence of bacteriophage fd DNA.

10. Use of short synthetic DNA duplexes as substrates for the restriction endonucleases Hpa II and Mno I.

1. Type II restriction endonucleases cleave single-stranded DNAs in general.

2. DNA denatures upon drying after ethanol precipitation.

3. The pathway of E. coli RNA polymerase-promoter complex formation as visualized by footprinting.

4. Analysis of DNA structural patterns and sequence organization at the larval cuticle locus in Drosophila melanogaster.

5. Analysis of chromatin structure and DNA sequence organization: use of the 1,10-phenanthroline-cuprous complex.

6. Identification and sequence of the initiation site for rat 45S ribosomal RNA synthesis.