Literature DB >> 6284682

Use of triton WR-1339 in cytochemical and biochemical characterization of phytolysosomes.

C Coulomb, P J Coulom, G Michel.   

Abstract

A new cytochemical assay of acid phosphatase activity employing Triton WR-1339, a non-ionic detergent, has been used to demonstrate the probable origin of the phytolysosome system and of the organization of the Golgi complex in the root meristem, Cucurbita pepo. The results were controlled biochemically by differential and linear density gradient ultracentrifugation of extracts from normal and Triton-treated roots. The various fractions were characterized by electron microscopy or by assay of marker enzymes.

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Year:  1982        PMID： 6284682     DOI： 10.1007/bf01041213

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  9 in total

1.  GLUTARALDEHYDE STABILIZATION AS AN AID TO GOLGI APPARATUS ISOLATION.

Authors:  D J MORRE; H H MOLLENHAUER; J E CHAMBERS
Journal:  Exp Cell Res       Date:  1965-06       Impact factor: 3.905

2.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.

Authors:  C DE DUVE; B C PRESSMAN; R GIANETTO; R WATTIAUX; F APPELMANS
Journal:  Biochem J       Date:  1955-08       Impact factor: 3.857

3.  Formation of triton WR 1339-filled rat liver lysosomes. I. Properties and intracellular distribution of [3H]triton WR 1339.

Authors:  R Henning; H Plattner
Journal:  Exp Cell Res       Date:  1975-09       Impact factor: 3.905

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Biochemical analysis of the pinocytotic process. 3. Subcellular distribution and metabolic effects of ( 3 H)Triton WR-1339.

Authors:  R Henning; H D Kaulen; W Stoffel
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1971-10

6.  Changes in lipid composition of triton-filled lysosomes during lysis. Association with activation of acid-active lipases and phospholipases.

Authors:  W B Weglicki; R C Ruth; K Owens; H D Griffin; B M Waite
Journal:  Biochim Biophys Acta       Date:  1974-01-23

Review 7.  Functions of lysosomes.

Authors:  C De Duve; R Wattiaux
Journal:  Annu Rev Physiol       Date:  1966       Impact factor: 19.318

8.  Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia, studied by thick section and thin section cytochemistry.

Authors:  P M Novikoff; A B Novikoff; N Quintana; J J Hauw
Journal:  J Cell Biol       Date:  1971-09       Impact factor: 10.539

9.  STUDIES ON THE INTRACELLULAR DIGESTIVE PROCESS IN MAMMALIAN TISSUE CULTURE CELLS.

Authors:  G B Gordon; L R Miller; K G Bensch
Journal:  J Cell Biol       Date:  1965-05-01       Impact factor: 10.539
  9 in total
  2 in total

1.  Amorphous areas in the cytoplasm of Dendrobium tepal cells: production through organelle degradation and destruction through macroautophagy?

Authors:  Wouter G van Doorn; Kanjana Kirasak; Saichol Ketsa
Journal:  Autophagy       Date:  2013-05-06       Impact factor: 16.016

2.  Classical macroautophagy in Lobivia rauschii (Cactaceae) and possible plastidial autophagy in Tillandsia albida (Bromeliaceae) tapetum cells.

Authors:  Alessio Papini; Stefano Mosti; Wouter G van Doorn
Journal:  Protoplasma       Date:  2013-10-25       Impact factor: 3.356
  2 in total

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