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Literature DB >> 6283470

Nucleotide sequence analysis of the linked left and right hand terminal regions of adenovirus type 5 DNA present in the transformed rat cell line 5RK20.

L Visser, A C Reemst, A D van Mansfeld, T H Rozijn.

Abstract

A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 transformed rat cell line 5RK20 has been cloned in the lambda vector Charon21A, including the segment containing the linked terminal regions. Sequence analysis of the linkage region showed a perfect homology with the Ad5 DNA sequence and a direct linkage of basepair (bp) 63 of the left hand end of the viral genome to bp 108 of the right hand end. No cellular or rearranged viral sequences were present. Our findings suggest that the joining of viral sequences into the cellular genome.

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Year: 1982 PMID： 6283470 PMCID： PMC320602 DOI： 10.1093/nar/10.7.2189

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

1. In vitro packaging of lambda and cosmid DNA.

Authors: B Hohn
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

2. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

Authors: L Wieslander
Journal: Anal Biochem Date: 1979-10-01 Impact factor: 3.365

10. The nucleotide sequence of the right-hand terminus of adenovirus type 5 DNA: implications for the mechanism of DNA replication.

Authors: P H Steenbergh; J S Sussenbach
Journal: Gene Date: 1979-08 Impact factor: 3.688

7 in total

1. Chromosomal integration pattern of a helper-dependent minimal adenovirus vector with a selectable marker inserted into a 27.4-kilobase genomic stuffer.

Authors: M Hillgenberg; H Tönnies; M Strauss
Journal: J Virol Date: 2001-10 Impact factor: 5.103

2. Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA.

Authors: K Van Doren; D Hanahan; Y Gluzman
Journal: J Virol Date: 1984-05 Impact factor: 5.103

3. Nucleotide sequence at the site of junction between adenovirus type 12 DNA and repetitive hamster cell DNA in transformed cell line CLAC1.

Authors: S Stabel; W Doerfler
Journal: Nucleic Acids Res Date: 1982-12-20 Impact factor: 16.971

4. Compilation of DNA strand exchange sites for non-homologous recombination in somatic cells.

Authors: A K Konopka
Journal: Nucleic Acids Res Date: 1988-03-11 Impact factor: 16.971

5. Integration of viral DNA into the genome of the adenovirus type 2-transformed hamster cell line HE5 without loss or alteration of cellular nucleotides.

Authors: R Gahlmann; W Doerfler
Journal: Nucleic Acids Res Date: 1983-11-11 Impact factor: 16.971

6. Covalently closed circles of human adenovirus DNA are infectious.

Authors: F L Graham
Journal: EMBO J Date: 1984-12-01 Impact factor: 11.598

7. Aedes aegypti insecticide resistance underlies the success (and failure) of Wolbachia population replacement.

Authors: Gabriela A Garcia; Ary A Hoffmann; Rafael Maciel-de-Freitas; Daniel A M Villela
Journal: Sci Rep Date: 2020-01-09 Impact factor: 4.379

7 in total

Nucleotide sequence analysis of the linked left and right hand terminal regions of adenovirus type 5 DNA present in the transformed rat cell line 5RK20.

1. In vitro packaging of lambda and cosmid DNA.

2. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

3. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

4. The nucleotide sequences at the termini of adenovirus-2 DNA.

5. The visualization of a circular DNA-protein complex from adenovirions.

6. The isolation of structural genes from libraries of eucaryotic DNA.

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

8. A physical map of the DNA regions flanking the rabbit beta-globin gene.

9. Construction of restriction enzyme fragment libraries containing DNA from human adenovirus types 2 and 5.