Literature DB >> 6282342

[Isolation, purification and properties of restriction endonuclease EcoRII].

V G Kosykh, S A Puntezhis, Ia I Bur'ianov, A A Baev.   

Abstract

The restriction endonuclease Eco RII was isolated and purified to homogeneity. The isolation procedure involved the use of the E. coli strain B834/pSK323, containing the recombinant plasmide pSK323 which provides for the oversynthesis of Eco RII enzymes. Data from gel filtration and Na-DS electrophoresis suggest that the restriction endonuclease Eco RII is a protein made up of two subunits, each with molecular weight of 44 000.

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Year:  1982        PMID： 6282342

Source DB:  PubMed          Journal:  Biokhimiia        ISSN： 0320-9725


  4 in total

1.  Direct monitoring of allosteric recognition of type IIE restriction endonuclease EcoRII.

Authors:  Shuntaro Takahashi; Hisao Matsuno; Hiroyuki Furusawa; Yoshio Okahata
Journal:  J Biol Chem       Date:  2008-03-26       Impact factor: 5.157

2.  Processive cleavage of concatemer DNA duplexes by Eco RII restriction endonuclease.

Authors:  A A Yolov; E S Gromova; Z A Shabarova
Journal:  Mol Biol Rep       Date:  1985-04       Impact factor: 2.316

3.  Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-strand cleavages.

Authors:  A A Yolov; E S Gromova; E A Kubareva; V K Potapov; Z A Shabarova
Journal:  Nucleic Acids Res       Date:  1985-12-20       Impact factor: 16.971

4.  Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. VI. The binding and cleavage of substrates containing nucleotide analogs.

Authors:  A A Yolov; M N Vinogradova; E S Gromova; A Rosenthal; D Cech; V P Veiko; V G Metelev; V G Kosykh; Y I Buryanov; A A Bayev
Journal:  Nucleic Acids Res       Date:  1985-12-20       Impact factor: 16.971
  4 in total

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