The effect of interscapular brown adipose tissue removal on body-weight and cold response in the mouse.

1. The removal of the interscapular brown adipose tissue (IBAT) led to an increase in body-weight of normal, lean mice as compared to anaesthetized controls. 2. No significant difference in food consumption could be detected between the two groups of mice over the period of the experiment. 3. Fat extraction of the whole carcasses with chloroform:methanol showed a statistically significant increase in fat content in the animals without IBAT. 4. There was no apparent failure in the operated animals to sustain core temperature when exposed to a cold stress situation (4 degrees for 24 h). 5. There was no difference in the wet weight, protein content or cytochrome oxidase content of the dorso-cervical brown adipose tissue (DCBAT) between operated and control mice. This is indicative of a lack of proliferation of other brown adipose tissue sites in the operated mice in response to the removal of the IBAT. 6. It is suggested that brown adipose tissue is implicated in dietary thermogenesis in the mouse.