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Literature DB >> 6281638

The role of histone H2B from sea urchin sperm in the association of reconstituted minichromosomes.

T N Osipova, V I Vorob'ev, M Böttger, C U von Mickwitz, S Scherneck.

Abstract

A comparative study of the condensation of reconstituted complexes of circular SV40 DNA with core histones from calf thymus and sea urchin sperm was performed using sedimentation and electron microscopic techniques. It is shown that in low ionic strength solutions both types of complexes are similar to native 'minichromosomes'. In the region from 0.08 to 0.16 M NaCl the complexes of SV40 DNA with thymus histones form small compact particles. By contrast, the compaction of the SV40 DNA complexes with sperm histones results in the formation of giant intermolecular associates. The results obtained may mean that histone H2B of sea urchin sperm participates in the formation of a higher order structure in sperm chromatin.

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Year: 1982 PMID： 6281638 DOI： 10.1007/bf00778507

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

13 in total

1. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

Authors: P Oudet; M Gross-Bellard; P Chambon
Journal: Cell Date: 1975-04 Impact factor: 41.582

2. A sedimentation study of the interaction of superhelical SV40 DNA with H1 histone.

Authors: M Böttger; S Scherneck; H Fenske
Journal: Nucleic Acids Res Date: 1976-02 Impact factor: 16.971

3. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.

Authors: R D Camerini-Otero; B Sollner-Webb; G Felsenfeld
Journal: Cell Date: 1976-07 Impact factor: 41.582

4. The multifunctional role of histone H1, probed with the SV40 minichromosome.

Authors: J D Griffith; G Christiansen
Journal: Cold Spring Harb Symp Quant Biol Date: 1978

Review 5. More histone structures.

Authors: C Von Holt; W N Strickland; W F Brandt; M S Strickland
Journal: FEBS Lett Date: 1979-04-15 Impact factor: 4.124

1. Acetylation and methylation patterns of core histones are modified after heat or arsenite treatment of Drosophila tissue culture cells.

Authors: A P Arrigo
Journal: Nucleic Acids Res Date: 1983-03-11 Impact factor: 16.971

1 in total

The role of histone H2B from sea urchin sperm in the association of reconstituted minichromosomes.

1. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

2. A sedimentation study of the interaction of superhelical SV40 DNA with H1 histone.

3. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.

4. The multifunctional role of histone H1, probed with the SV40 minichromosome.

Review 5. More histone structures.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Studies on histones. 7. Preparative methods for histone fractions from calf thymus.

8. Complexes of DNA with arginine-rich and slightly lysine-rich histones. Transcription and electron microscopy.

9. An octamer of core histones in solution: central role of the H3-H4 tetramer in the self-assembly.

10. Salt-and histone H1-induced structural changes of reconstituted minichromosomes.

1. Acetylation and methylation patterns of core histones are modified after heat or arsenite treatment of Drosophila tissue culture cells.