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Literature DB >> 6280825

A "liver" antigen associated with avian erythroblastosis: binding by bentonite and precipitation with sodium dodecyl sulphate.

Abstract

The properties of a complement fixing antigen, EbAg, extracted from erythroblastosis-affected chicken livers are described. The antigen in extracts freed of structural protein is strongly bound by bentonite, but not by barium sulphate. Strongly alkaline solutions of sodium dodecyl sulphate are required to release the antigen from bentonite. Acidification of the detergent solution precipitates the active solution precipitates the active protein. Extraction of heme from the acidified detergent precipitate by methyl-ethyl ketone further purifies the antigen. This acid detergent treatment eliminates the need to use bentonite as a purification step.

Entities: Chemical Disease Species

Mesh：

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Year: 1982 PMID： 6280825 PMCID： PMC1320204

Source DB: PubMed Journal: Can J Comp Med ISSN： 0008-4050

9 in total

1. AN AVIAN LEUCOSIS GROUP-SPECIFIC COMPLEMENT FIXATION REACTION. APPLICATION FOR THE DETECTION AND ASSAY OF NON-CYTOPATHOGENIC LEUCOSIS VIRUSES.

Authors: P S SARMA; H C TURNER; R J HUEBNER
Journal: Virology Date: 1964-07 Impact factor: 3.616

2. Adsorption of serum lipids by montmorillonite.

Authors: E NIKKILA; N OKER-BLOM
Journal: Science Date: 1952-12-19 Impact factor: 47.728

Review 3. Cell-surface antigens induced by RNA tumour viruses.

Authors: R Kurth; E M Fenyö; E Klein; M Essex
Journal: Nature Date: 1979-05-17 Impact factor: 49.962

4. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. III. Avian viral proteins with group-specific antigenicity.

Authors: P Fletcher; R C Nowinski; E Tress; E Fleissner
Journal: Virology Date: 1975-04 Impact factor: 3.616

1 in total

Review 1. Reflections on the pathogenesis of diseases caused by the acute avian leukosis/sarcoma viruses with special reference to avian erythroblastosis.

Authors: C Darcel
Journal: Vet Res Commun Date: 1994 Impact factor: 2.459

1 in total

A "liver" antigen associated with avian erythroblastosis: binding by bentonite and precipitation with sodium dodecyl sulphate.

1. AN AVIAN LEUCOSIS GROUP-SPECIFIC COMPLEMENT FIXATION REACTION. APPLICATION FOR THE DETECTION AND ASSAY OF NON-CYTOPATHOGENIC LEUCOSIS VIRUSES.

2. Adsorption of serum lipids by montmorillonite.

Review 3. Cell-surface antigens induced by RNA tumour viruses.

4. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. III. Avian viral proteins with group-specific antigenicity.

5. A complement fixing antigen in the livers of birds infected with an avian leukovirus (erythroblastosis virus).

6. Recombination in the avian oncoviruses as a model for the generation of defective transforming viruses.

7. The removal of "nucleic acid" from an antigen present in the livers of chickens with erythroblastosis.

8. Avian oncovirus proteins expressed on the surface of infected cells.

9. Viral polyproteins in chick embryo fibroblasts infected with avian sarcoma leukosis viruses.

Review 1. Reflections on the pathogenesis of diseases caused by the acute avian leukosis/sarcoma viruses with special reference to avian erythroblastosis.