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Literature DB >> 6279055

The genome of orf virus: restriction endonuclease analysis of viral DNA isolated from lesions of orf in sheep.

Abstract

The purification of orf virus directly from scab material from clinical cases of orf in sheep and restriction endonuclease analysis of the viral DNA is described. Between 7 x 10(9) and 1.6 x 10(11) virus particles, and 0.7 to 22.8 micrograms of viral DNA could be recovered from 1g of scab material. Considerable heterogeneity was observed between different field isolates when restriction endonuclease digests of orf DNA were compared by gel electrophoresis. It was also shown, for two isolates, that these fragment patterns did not change after plaque purification and passage in cell culture. It is suggested that restriction endonuclease analysis of viral DNA offers a convenient method of identification of isolates of orf virus. The molecular weight of orf DNA was determined and found to be 88.8 x 10(6).

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Year: 1982 PMID： 6279055 DOI： 10.1007/bf01315174

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

23 in total

1. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.

Authors: K Murray; N E Murray
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus.

Authors: J D Gangemi; D G Sharp
Journal: J Virol Date: 1976-10 Impact factor: 5.103

3. THE STRUCTURE OF ORF VIRUS.

Authors: J NAGINGTON; A A NEWTON; R W HORNE
Journal: Virology Date: 1964-08 Impact factor: 3.616

4. Morphological studies of orf and vaccinia viruses.

Authors: J NAGINGTON; R W HORNE
Journal: Virology Date: 1962-03 Impact factor: 3.616

5. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

6. Isolation and comparative study "in vitro" of five strains of contagious ecthyma of sheep.

Authors: P Precausta; C Stellmann
Journal: Zentralbl Veterinarmed B Date: 1972-07

7. Mature form of the deoxyribonucleic acid from chick embryo lethal orphan virus.

Authors: H B Younghusband; A J Bellett
Journal: J Virol Date: 1971-09 Impact factor: 5.103

8. Genetic and antigenic heterogeneity of different parapoxvirus strains.

Authors: R Wittek; M Herlyn; D Schümperli; P A Bachmann; A Mayr; R Wyler
Journal: Intervirology Date: 1980 Impact factor: 1.763

9. Differentiation of sub-types of equine herpesvirus I by restriction endonuclease analysis.

Authors: M Sabine; G R Robertson; J M Whalley
Journal: Aust Vet J Date: 1981-03 Impact factor: 1.281

10. Studies on the virus of Ecthyma contagiosum. 3. Multiplicity of virus strains.

Authors: A N Sawhney
Journal: Izv Mikrobiol Inst (Sofiia) Date: 1966

26 in total

1. Activities of acyclic nucleoside phosphonates against Orf virus in human and ovine cell monolayers and organotypic ovine raft cultures.

Authors: F Dal Pozzo; G Andrei; A Holy; J Van Den Oord; A Scagliarini; E De Clercq; R Snoeck
Journal: Antimicrob Agents Chemother Date: 2005-12 Impact factor: 5.191

9. Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep.

Authors: P Chand; R P Kitching; D N Black
Journal: Epidemiol Infect Date: 1994-10 Impact factor: 2.451

10. Orf virus replication in bovine testis cells: kinetics of viral DNA, polypeptide, and infectious virus production and analysis of virion polypeptides.

Authors: T C Balassu; A J Robinson
Journal: Arch Virol Date: 1987 Impact factor: 2.574

The genome of orf virus: restriction endonuclease analysis of viral DNA isolated from lesions of orf in sheep.

1. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.

2. Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus.

3. THE STRUCTURE OF ORF VIRUS.

4. Morphological studies of orf and vaccinia viruses.

5. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

6. Isolation and comparative study "in vitro" of five strains of contagious ecthyma of sheep.

7. Mature form of the deoxyribonucleic acid from chick embryo lethal orphan virus.

8. Genetic and antigenic heterogeneity of different parapoxvirus strains.

9. Differentiation of sub-types of equine herpesvirus I by restriction endonuclease analysis.

10. Studies on the virus of Ecthyma contagiosum. 3. Multiplicity of virus strains.

1. Activities of acyclic nucleoside phosphonates against Orf virus in human and ovine cell monolayers and organotypic ovine raft cultures.

2. Analysis of parapoxvirus genomes.

3. Comparison of contagious ecthyma virus genomes by restriction endonucleases.

4. A homolog of interleukin-10 is encoded by the poxvirus orf virus.

5. A novel Bcl-2-like inhibitor of apoptosis is encoded by the parapoxvirus ORF virus.

6. Genomes of the parapoxviruses ORF virus and bovine papular stomatitis virus.

7. Orf virus and vaccinia virus do not cross-protect sheep.

8. Homologs of vascular endothelial growth factor are encoded by the poxvirus orf virus.

9. Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep.

10. Orf virus replication in bovine testis cells: kinetics of viral DNA, polypeptide, and infectious virus production and analysis of virion polypeptides.