Literature DB >> 6278448

Separation of closed circular DNA from linear DNA by electrophoresis in two dimensions in agarose gels.

A Oppenheim.   

Abstract

A method that provides an easy, rapid, and reproducible way for separating closed circular DNA species from linear DNA and nicked circles is described. The method is based on the difference in mobility of form I (supercoiled) DNA and form II (nicked circles), and the differential mobility of relaxed circular DNA in the presence and absence of ethidium bromide (EtdBr). It can be used for detection or for purification of plasmid, episomal, or viral DNA from the bulk of cellular DNA, or from other DNA mixtures.

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Year:  1981        PMID： 6278448      PMCID： PMC327643          DOI： 10.1093/nar/9.24.6805

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  The sense of naturally occurring superhelices and the unwinding angle of intercalated ethidium.

Authors:  D E Pulleyblank; A R Morgan
Journal:  J Mol Biol       Date:  1975-01-05       Impact factor: 5.469

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1974-11-15       Impact factor: 5.469

4.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

5.  Terminal stages of SV40 DNA replication proceed via multiply intertwined catenated dimers.

Authors:  O Sundin; A Varshavsky
Journal:  Cell       Date:  1980-08       Impact factor: 41.582

6.  Analysis of DNA double- and single-strand breaks by two dimensional electrophoresis: action of micrococcal nuclease on chromatin and DNA, and degradation in vivo of lens fiber chromatin.

Authors:  S P Modak; P Beard
Journal:  Nucleic Acids Res       Date:  1980-06-25       Impact factor: 16.971

7.  Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.

Authors:  W Keller
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

8.  Nicking-closing activity associated with bacteriophage lambda int gene product.

Authors:  Y Kikuchi; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

9.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences.

Authors:  M F Law; D R Lowy; I Dvoretzky; P M Howley
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205
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  12 in total

1.  Involvement of a plasmid in growth on and dispersion of crude oil by Acinetobacter calcoaceticus RA57.

Authors:  S Rusansky; R Avigad; S Michaeli; D L Gutnick
Journal:  Appl Environ Microbiol       Date:  1987-08       Impact factor: 4.792

2.  Inactivation of topoisomerase I or II may lead to recombination or to aberrant replication termination on both SV40 and yeast 2 micron DNA.

Authors:  P Levac; T Moss
Journal:  Chromosoma       Date:  1996-10       Impact factor: 4.316

3.  CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects.

Authors:  Karthik Murugan; Arun S Seetharam; Andrew J Severin; Dipali G Sashital
Journal:  J Biol Chem       Date:  2020-03-11       Impact factor: 5.157

4.  Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus.

Authors:  C L Keen; S Mendelovitz; G Cohen; Y Aharonowitz; K L Roy
Journal:  Mol Gen Genet       Date:  1988-04

5.  Induction of circles of heterogeneous sizes in carcinogen-treated cells: two-dimensional gel analysis of circular DNA molecules.

Authors:  S Cohen; S Lavi
Journal:  Mol Cell Biol       Date:  1996-05       Impact factor: 4.272

6.  Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR-Cas9 variants.

Authors:  Karthik Murugan; Shravanti K Suresh; Arun S Seetharam; Andrew J Severin; Dipali G Sashital
Journal:  Nucleic Acids Res       Date:  2021-04-19       Impact factor: 16.971

7.  Cloning and nucleotide sequence of a linear DNA plasmid from Xanthophyllomyces dendrorhous (Phaffia rhodozyma).

Authors:  L M Santopietro; M R Kula
Journal:  Folia Microbiol (Praha)       Date:  2001       Impact factor: 2.099

8.  Isotopic labeling experiments that elucidate the mechanism of DNA strand cleavage by the hypoxia-selective antitumor agent 1,2,4-benzotriazine 1,4-di-N-oxide.

Authors:  Xiulong Shen; Anuruddha Rajapakse; Fabio Gallazzi; Venkatraman Junnotula; Tarra Fuchs-Knotts; Rainer Glaser; Kent S Gates
Journal:  Chem Res Toxicol       Date:  2013-12-19       Impact factor: 3.739

9.  Osmophilic linear plasmids from the salt-tolerant yeast Debaryomyces hansenii.

Authors:  N Gunge; K Fukuda; S Morikawa; K Murakami; M Takeda; A Miwa
Journal:  Curr Genet       Date:  1993 May-Jun       Impact factor: 3.886

10.  Possible chemical mechanisms underlying the antitumor activity of S-deoxyleinamycin.

Authors:  Santhosh Sivaramakrishnan; Kent S Gates
Journal:  Bioorg Med Chem Lett       Date:  2007-11-28       Impact factor: 2.823
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