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Literature DB >> 6277871

Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion.

Abstract

A phoR strain was constructed with transposon Tn10 inserted near the phoM+ locus. This was done without any prior knowledge of the phoM map location. Subsequently, we defined the phoM map position by screening tetracycline-sensitive (Tcs) derivatives for mutants which were both alkaline phosphatase negative (ther phoR phoM double mutant phenotype) and auxotrophic simultaneously. Some of these mutants were Thr-. Bacteriophage P1-mediated transductions were used to confirm that phoM and its nearby Tn10 insertion were closely linked to thr. Unexpectedly, 7 of 10 mutants analyzed also had mutations unlinked to the phoM-thr-Tn10 region. These may represent a new type of Tn10-promoted molecular event which is caused by transposition of a Tn10 end (IS10).

Entities: Chemical Gene Species

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Year: 1982 PMID： 6277871 PMCID： PMC220136 DOI： 10.1128/jb.150.1.429-432.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

1. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins.

Authors: B L Wanner; A Sarthy; J Beckwith
Journal: J Bacteriol Date: 1979-10 Impact factor: 3.490

2. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.

Authors: N Kleckner; D F Barker; D G Ross; D Botstein
Journal: Genetics Date: 1978-11 Impact factor: 4.562

3. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10.

Authors: N Kleckner; K Reichardt; D Botstein
Journal: J Mol Biol Date: 1979-01-05 Impact factor: 5.469

4. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.

Authors: N Kleckner; J Roth; D Botstein
Journal: J Mol Biol Date: 1977-10-15 Impact factor: 5.469

5. Physical structures of Tn10-promoted deletions and inversions: role of 1400 bp inverted repetitions.

Authors: D G Ross; J Swan; N Kleckner
Journal: Cell Date: 1979-04 Impact factor: 41.582

6. Culture medium for enterobacteria.

Authors: F C Neidhardt; P L Bloch; D F Smith
Journal: J Bacteriol Date: 1974-09 Impact factor: 3.490

7. Electron microscopy of polar insertions in the lac operon of Escherichia coli.

Authors: M H Malamy; M Fiandt; W Szybalski
Journal: Mol Gen Genet Date: 1972

8. Amber mutants of the trpR regulatory gene.

Authors: D E Morse; C Yanofsky
Journal: J Mol Biol Date: 1969-08-28 Impact factor: 5.469

9. Localized mutagenesis of any specific small region of the bacterial chromosome.

Authors: J S Hong; B N Ames
Journal: Proc Natl Acad Sci U S A Date: 1971-12 Impact factor: 11.205

10. Fine structure analysis of the threonine operon in Escherichia coli K-12.

Authors: I Saint-Girons; D Margarita
Journal: Mol Gen Genet Date: 1978-06-01

12 in total

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors: M K Berlyn
Journal: Microbiol Mol Biol Rev Date: 1998-09 Impact factor: 11.056

2. Regulation of ugp, the sn-glycerol-3-phosphate transport system of Escherichia coli K-12 that is part of the pho regulon.

Authors: H Schweizer; W Boos
Journal: J Bacteriol Date: 1985-07 Impact factor: 3.490

7. Identification of the dye gene product, mutational loss of which alters envelope protein composition and also affects sex factor F expression in Escherichia coli K-12.

Authors: R S Buxton; L S Drury
Journal: Mol Gen Genet Date: 1984

Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion.

1. Escherichia coli pleiotropic mutant that reduces amounts of several periplasmic and outer membrane proteins.

2. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.

3. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10.

4. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.

5. Physical structures of Tn10-promoted deletions and inversions: role of 1400 bp inverted repetitions.

6. Culture medium for enterobacteria.

7. Electron microscopy of polar insertions in the lac operon of Escherichia coli.

8. Amber mutants of the trpR regulatory gene.

9. Localized mutagenesis of any specific small region of the bacterial chromosome.

10. Fine structure analysis of the threonine operon in Escherichia coli K-12.

Review 1. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

2. Regulation of ugp, the sn-glycerol-3-phosphate transport system of Escherichia coli K-12 that is part of the pho regulon.

Review 3. Linkage map of Escherichia coli K-12, edition 7.

4. Cloning and characterization of the alkaline phosphatase positive regulatory gene (phoM) of Escherichia coli.

5. Bacterial alkaline phosphatase clonal variation in some Escherichia coli K-12 phoR mutant strains.

6. Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon.

7. Identification of the dye gene product, mutational loss of which alters envelope protein composition and also affects sex factor F expression in Escherichia coli K-12.

8. D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.

9. Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region.

10. Molecular cloning of the wild-type phoM operon in Escherichia coli K-12.