Literature DB >> 6277404

A comparison of resolution-enhancement methods in saturation-transfer EPR. 15N isotopically substituted spin labels and 35 GHz high-frequency operation.

M E Johnson, P Thiyagarajan, B Bates, B L Currie.   

Abstract

The use of (15)N isotopic substitution and 35 GHz (Q-band) operation as resolution enhancement methods in saturation-transfer electron paramagnetic resonance (ST-EPR) are compared. We find that both methods offer roughly comparable enhancements in ST-EPR resolution. The most powerful approach to resolving complex ST-EPR spectral behavior, however, will probably be the combined use of multiple-frequency X- and Q-band operation with (15)N isotopically substituted spin labels.

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Year:  1982        PMID： 6277404      PMCID： PMC1328836          DOI： 10.1016/s0006-3495(82)84700-0

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  23 in total

1.  Minor hemoglobins in sickle cell anemia, beta-thalassemia, and related conditions: a study of red cell fractions isolated by density gradient centrifugation.

Authors:  E C Abraham; D Walker; M Gravely; T H Huisman
Journal:  Biochem Med       Date:  1975-05

2.  Spin-label studies of protein-protein interactions in retinal rod outer segment membranes. Saturation transfer electron paramagnetic resonance spectroscopy.

Authors:  A Baroin; A Bienvenue; P F Devaux
Journal:  Biochemistry       Date:  1979-04-03       Impact factor: 3.162

3.  Rotational motion of the sarcoplasmic reticulum Ca2+-ATPase.

Authors:  D D Thomas; C Hidalgo
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

4.  Saturation transfer electron spin resonance study on the rotational diffusion of calcium- and magnesium-dependent adenosine triphosphatase in sarcoplasmic reticulum membranes.

Authors:  Y Kirino; T Ohkuma; H Shimizu
Journal:  J Biochem       Date:  1978-07       Impact factor: 3.387

5.  Saturation-transfer spectroscopy.

Authors:  J S Hyde
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

6.  Librational motion of an "immobilized" spin label: hemoglobin spin labeled by a maleimide derivative.

Authors:  M E Johnson
Journal:  Biochemistry       Date:  1978-04-04       Impact factor: 3.162

7.  Molecular dynamics of spectrin-actin at low pH: saturation transfer EPR studies.

Authors:  L W Fung; M J Soo Hoo; W A Meena
Journal:  FEBS Lett       Date:  1979-09-15       Impact factor: 4.124

8.  Effect of the lipid environment on protein motion and enzymatic activity of sarcoplasmic reticulum calcium ATPase.

Authors:  C Hidalgo; D D Thomas; N Ikemoto
Journal:  J Biol Chem       Date:  1978-10-10       Impact factor: 5.157

9.  New EPR methods for the study of very slow motion: application to spin-labeled hemoglobin.

Authors:  J S Hyde; D D Thomas
Journal:  Ann N Y Acad Sci       Date:  1973-12-31       Impact factor: 5.691

10.  Use of sodium cyanoborohydride in the preparation of biologically active nitroxides.

Authors:  G M Rosen
Journal:  J Med Chem       Date:  1974-03       Impact factor: 7.446
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  2 in total

1.  High field/high frequency saturation transfer electron paramagnetic resonance spectroscopy: increased sensitivity to very slow rotational motions.

Authors:  Eric J Hustedt; Albert H Beth
Journal:  Biophys J       Date:  2004-06       Impact factor: 4.033

2.  Distance and dynamics determination by W-band DEER and W-band ST-EPR.

Authors:  Likai Song; Mioara Larion; Jean Chamoun; Marco Bonora; Piotr G Fajer
Journal:  Eur Biophys J       Date:  2009-12-09       Impact factor: 1.733
  2 in total

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